海藻油微胶囊中DHA检测预处理方法的比较

摘要

The algal oil was microcapsulated with gum/casein and modified starch/sugar as two sets of wall material. The DHA of microcapsulated algal oil was determined by breaking wall material first with enzymic and supersonic treatment separately, and then extracting oil with chloroform-methanol, alky aether and acid hydrolysis method. The DHA content was analyzed by gas chromatography after lipids methylated. It showed that the determined results were effected with wall-material broken method and oil extraction method for the same sample. The DHA content with alky-aether oil extraction was higher than others after the same wall-material broken treatment; Using the same oil extraction method, the DHA content with enzymic was higher than supersonic treatment.%对糊精、酪蛋白与变性淀粉、蔗糖两组壁材组合制备的海藻油微胶囊,采用酶法破壁与超声波法破壁,然后分别用氯仿-甲醇法、碱性乙醚法和酸水解法提取油脂,甲脂化后气相色谱分析二十二碳六烯酸(DHA)含量.结果表明:对于同一种微胶囊样品,破壁方法和油脂提取方法的不同均会对DHA的含量测定有影响.同一种破壁方法,采用碱性乙醚法提取油脂测得的DHA含量较其它方法高;同一种油脂提取方法,酶法破壁测得的DHA含量比超声波法测得的含量高.