Based on colloidal gold immunochromatography assay to detect the content of nitrofuran metabolites in aquatic products, and study on sample pretreatment. The results showed that the best derivatizing agent was 4-nitrobenzaldehyde (4-NP), dosage was 0.1 mL. Derivative conditions was 60℃water bath conditions incubated for 60 min.The dosage of extractant and purifying agent were 6.0 and 1.0 mL. The linear range of AOZ,SEM, AMOZ,AHD were 0.0μg/L-4.0μg/L,0.0μg/L-4.0μg/L,0.0μg/L-4.0μg/L,0.0μg/L-4.0μg/L. The recovery rate and the relative standard deviation were 76.6%-102.3%and 3.03%-7.40%. This sample pre-treatment method was suitable for the the nitrofuran metabolites immune colloidal gold rapid detection.%采用胶体金免疫层析方法建立了水产品中硝基呋喃类代谢物的快速检测,并对其样品前处理方法进行了研究。结果表明,最佳衍生剂为4-硝基苯甲醛(4-NP),用量为0.1 mL,衍生条件为60℃水浴条件下孵育60 min,提取剂用量为6.0 mL,净化剂用量为1.0 mL。呋喃唑酮代谢物(AOZ)、呋喃西林代谢物(SEM)、呋喃它酮代谢物(AMOZ)、呋喃妥因代谢物(AHD)的线性范围分别为0.0μg/L~4.0μg/L、0.0μg/L~4.0μg/L、0.0μg/L~6.0μg/L、0.0μg/L~6.0μg/L;添标回收率均为76.6%~102.3%;相对标准偏差均为3.03%~7.40%。该样本前处理方法适合硝基呋喃类代谢物免疫胶体金快速检测。
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