摘要：The effects of liquid medium pollen, including pure pollen of Yunyan 85, Yunyan 87 and K326 and boric acid, sucrose, calcium chloride, Put, Spd and Spm solution, on the fruit setting, seed vigor and seedling quality of MS Yunyan 85, MS Yunyan 87 and MS K326 were investigated, with the pure pollen as the control. The results showed that the effects of artificial pollination with the liquid medium pollen which contained 0. 01 g calcium chloride, 100 g sucrose and 100 g pure pollen per liter was better for MS Yunyan 85, the fruit setting percentage increased by 19. 98％, the 1 000-grain weight increased 2 mg, seedling dry weight increased 1.7 mg, the amount of pure pollen used decreased by 37. 6％, the liquid medium pollen had no significant effect on seed number per capsule, seed weight per capsule, germination energy, germination percentage, germination index and mean germination time. For MS Yunyan 87 and MS K326, the effects of artificial pollination with liquid medium pollen containing 0.5 mmol Spd and 150 g pure pollen per liter was better, the 1 000-grain weight increased 7.4 mg and 8.4 mg respectively, dry weight of seedlings increased 0. 95 mg and0. 15 mg respectively, and fruit setting percentage of MS K326 increased by 26. 09％, the pure pollen amount decreased by 40. 1％ and 39.3％, and had no significant effect on other indexes. The results indicated that the liquid medium pollen used in tobacco seed production could save the application amount of pure pollen on the basis of ensuring the yield and quality of seed, thus reducing the seed production costs.%将硼酸、蔗糖、CaCl2、Put、Spd和Spm溶液与纯花粉混合,制成不同花粉比例的液体介质花粉,以纯花粉为对照,研究介质花粉对‘MS云烟85'、‘MS云烟87'和‘MS K326'的坐果、种子活力及幼苗干重的影响.结果表明:用(0.01 g CaCl2﹢100 g蔗糖+100 g纯花粉)·L-1的液体介质花粉对‘MS云烟85'授粉,坐果率较对照提高19.98%,种子千粒重及幼苗干重分别增加2.0 mg和1.7 mg,纯花粉用量减少37.6%,且未影响每蒴果种子数、每蒴果种子重、种子发芽势、发芽率、发芽指数及平均发芽时间,授粉效果好.用(0.5 mmol Spd+150 g纯花粉)·L-1的液体介质花粉对‘MS云烟87'和‘MS K326'授粉,种子千粒重分别增加7.4 mg和8.4 mg,幼苗干重分别增加0.95 mg和0.15 mg,且‘MS K326'的坐果率提高了26.09%,授粉对两品种的其他各指标无显著影响,但花粉用量分别减少40.1%和39.3%.结果表明,在保证种子产量和质量的基础上,应用液体介质花粉可达到提高授粉效率,降低制种成本的目的.