红曲色素固态发酵优化研究

摘要

以色价和桔霉素为指标,通过正交实验对红曲霉AS3.531固态发酵产红曲色素的发酵条件进行了优化研究.结果表明：装料量60g/250mL、温度37℃、pH 5.5、培养基初始含水量70%时色价达到最高；装料量40g/250mL、温度37℃、pH 5.0、培养基初始含水量60%时,桔霉素的生物合成量最低,综合考虑高色价和低桔霉素,最优发酵条件为装料量50g/250mL、温度37℃、pH 5.0、培养基初始含水量70%,此条件下色价为256U/g,桔霉素含量为0.0805μg/g.%Choosing the pigment yield and citrinin as indexes, the conditions of the solid fermentation of Monascus AS3.531 producing Monascus pigment were optimized by orthogonal test. The results show that: fermentation condi-tions were that rice 60 g/250 mL, 37℃, pH 5.5, water content 70%, the yield of pigment is highest; fermentation conditions were that rice 40 g/250 mL, 37℃, pH 5.0, water content 60%, the biosynthesis amount citrinin is low-est. Considering the high color and low citrinin, the optimal conditions were found to be: rice 50 g/250 mL, 37℃, pH 5.0, water content 70%, under these conditions, the yield of citrinin is 0.0805μg/g, and pigment is 256 U/g.