黑豆低聚肽的抗氧化活性评价及其氨基酸组成分析

摘要

通过测定黑豆低聚肽在体外化学模拟体系中的总还原力、清除自由基活性及小鼠肝匀浆MDA的抑制能力来评价其抗氧化能力,并分析其氨基酸组成.结果表明:黑豆低聚肽的还原能力是Vc的0.714倍;黑豆低聚肽具有较强的清除自由基作用,清除O_2~-、DPPH·和·0H的IC_(50)值分别为1.32、0.67和0.96 mg/mL,抑制能力由大到小依次为DPPH·＞·OH＞O_2~-,分别是V_c IC_(50)值的1.07、1.15和1.76倍.黑豆低聚肽还能有效抑制羟自由基引起的脂质过氧化和小鼠肝匀浆MDA生成.%The free radical scavenging ability in chemically modeled systems and the restraining ability of raalon-dialdehyde (MDA) production in mice liver homogenate were used to estimate the antioxidant capabilities of black soybean oligopeptides. It shows that the half inhibit concentration ( IC_(50)) of oligopeptides for scavenging superoxide anion,DPPH ·,and hydroxyl radicals were 1.32,0.67 and 0.96 mg/mL,respectively,which were 1.07,1. 15,and 1. 76 times that of ascorbic acid. The reduction ability of ascorbic acid was 1.4 times that of oligopeptides. The black soybean oligopeptides exerted the strongest free radical scavenging activity. The black soybean oligopeptides can inhibit lipid peroxidation induced by · OH,as well as the formation of MDA in mice liver homogenate,showing that they possess a strong antioxidation capability.