低聚木糖-脯氨酸美拉德反应衍生物的制备及其抗氧化性能

摘要

以低聚木糖作为羰基供体与脯氨酸进行美拉德反应,监测反应过程中pH、紫外-可见吸收值以及荧光值,提取反应5h、15h以及30 h的低聚木糖衍生物XP-5h、XP-15h和XP-30h.对3种衍生物进行红外表征和相对分子质量测定,并研究其对DPPH自由基、超氧阴离子自由基(O2-·)的清除能力以及还原能力.结果表明:低聚木糖衍生物对DPPH自由基、O2-·的清除能力以及还原能力均较低聚木糖得到显著提高;XP-30h的抗氧化性最强,XP-15h次之,XP-5h最弱,即随着反应的进行低聚木糖衍生物的抗氧化性能增强.%Maillard reaction was occurred by heating xylo-oligosaccharide (XO) with proline.The pH,UV-Vis absorbance and fluorescence were determined during the reaction.Three kinds of xylo-oligosaccharide derivatives were extracted and named as XP-5h,XP-15h and XP-30h.Xylo-oligosaccharide derivatives were characterized by FTIR and their molecular weight was determined by GPC.Their antioxidant activity was investigated through various established in vitro systems,such as its ability to scavenge and reduce 1,1-diphenyl-2-pivrylhydrazyl(DPPH) and superoxide anion radicals.The results indicated that three kinds of xylo-oligosaccharide derivatives showed stronger antioxidant activities compared with xylo-oligosaccharides.The antioxidant activities were found to be in the order of XP-30h ＞ XP-15h ＞ XP-5h,which indicated that the antioxidant activity of xylo-oligosaccharide derivatives was enhanced during the process of the reaction.