LC-ESI-M S／M S方法测定人血浆肌苷的应用研究

摘要

Objective To develop a LC-MS/MS method for the determination of inosine in human plasma and to apply it in the isoprinosine pharmacokinetic researches .Methods With adefovir as the internal standard ,methanol -10 mmol/L ammonium ace-tate(15 :85 ,v/v) was adopted as the mobile phase .The chromatographic column was the Agilent SB-C18 column(5 μm ,150 mm × 4 .6 mm I .D .) .Electrospray ionization(ESI) source was applied and the detection was conducted by the multiple-reaction monito-ring(MRM) mode .The ion reactions for the quantitative analysis were m/z 135 .0-267 .3(inosine) and m/z 134 .1-272 .0(adefo-vir) .Results The linear range of plasma inosine was 10-3 000 ng/mL .The lower detection limit was 10 ng/mL .The inter-and in-tra-day precisions(RSD) were less than 5% .The average recovery rate was above 90% without the matrix effect .Conclusion This method has the strong specificity ,convenience in treating the sample and high sensitivity ,and is suitable for the inosine pharmacokinetic re-searches .%目的：建立LC-M S/M S测定人血浆中肌苷浓度的方法并应用于异丙肌苷药代动力学研究。方法以阿德福韦为内标，采用甲醇∶10 mmol/L 乙酸铵（15∶85，v/v ）为流动相，以Agilent SB-C18柱（5μm粒径，150．0 mm ×4．6 mm I ．D ．）为分析柱，通过电喷雾电离源（ESI），M RM扫描方式进行检测。用于定量分析的离子反应分别为m/z母离子为267．3，子离子为135．0（肌苷）和m/z母离子为272．0，子离子为134．1（阿德福韦）。结果血浆中肌苷定量的线性范围10～3000 ng/m L ，定量下限为10 ng/mL。日内、日间精密度（RSD）小于5％，平均回收率大于90％，无基质效应。结论本法专属性强，样品处理方便，灵敏度高，适用于肌苷临床药动学研究。