Aim To observe the effect of gypenosides ( GP) on the expression of receptor for advanced gly-cated endproducts ( RAGE ) and transforming growth factor-β1 ( TGF-β1 ) in human mesangial cells( HMCs) induced by AGEs. Methods HMCs were cultured in DMEM of low glucose containing 15% fetal bovine ser-um in vitro, which were divided into four groups: the normal group, model group, GP group and positive control group. In addition to the normal group, the other groups were stimulated by AGEs ( 200 mg · L-1 );furthermore, GP group was intervened with dif-ferent concentrations(25,75,175 mg·L-1) of GP, while control group was given 10 mmol · L-1 of amin-oguanidine hydrochloride. The expression of RAGE and TGF-β1 protein of each group was detected by Western blot; the expression of RAGE and TGF-β1 mRNA of each group was detected by RT-PCR. Re-sults The expression of RAGE, TGF-β1 protein and mRNA in HMCs induced by AGEs in the model group was significantly higher than that of the normal group ( P<0. 01 );compared with the positive control group ( P<0. 01 ) , GP could obviously reduce the expression of RAGE, TGF-β1 protein and mRNA in a dose-de-pendent manner. Conclusion GP can reduce the ex-pression of RAGE in HMCs induced by AGEs, block AGEs-RAGE signaling pathway and decrease the ex-pression of the downstream factor TGF-β1 , therefore, it plays the role in the resistance of rennal fibrosis in DN.%目的：观察绞股蓝皂苷( gypenosides,GP)对晚期糖基化终末产物( advanced glycation endproducts,AGEs)诱导下人肾小球系膜细胞( human mesangial cells,HMCs)中晚期糖基化终末产物受体( RAGE)及转化生长因子-β1( TGF-β1)表达的影响。方法将体外培养的(体积分数为0．15的FBS的DMEM低糖培养液)人肾小球系膜细胞( HMCs )分为4组：正常组、模型组、GP组、阳性对照组。除正常组外,其余组均再给予200 mg·L-1 AGEs 刺激。此外,GP组中加入不同浓度GP(25、75、175 mg·L-1)进行干预,阳性对照组中加入氨基胍盐酸盐(10-1 mmol·L-1)。应用Western blot技术检测各实验组中 RAGE和 TGF-β1蛋白的表达；RT-PCR技术检测各实验组中RAGE和TGF-β1 mRNA的表达。结果模型组AGEs诱导下HMCs中RAGE、TGF-β1蛋白及mRNA表达水平明显高于正常组( P <0．01)；与模型组比较, GP 组中GP可明显下调HMCs中RAGE、TGF-β1蛋白及mRNA的表达,且呈浓度依赖性(P<0．01)。结论 GP可降低AGEs诱导下 HMCs中 RAGE的表达,阻断 AGEs-RAGE 信号通路,并下调下游因子TGF-β1的表达,进而延缓糖尿病肾病纤维化进程。
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