3株海洋红酵母的分离及营养成分分析

摘要

In order to explore marine red yeasts as the nutritional value of aquatic baits for isolation and analysis of nutritional compositions of red yeasts from sea mud and seawater around Dongji Island,China.The isolates were identified by the observation of the morphology and the analysis of ribosomas ITS sequences.The contents of fatty acids and amino acids were analyzed.Results showed that the strains RI21 and DONG17 were identified as Rhodotorula mucilaginosa,and DONG45 as Rhodosporidim paludigenum.The main fatty acids of the three marine red yeasts were C16∶0,C18∶0,C18∶1 (n-9) and C18∶2 (n-6),and the total amino acid contents of the three strains RI21,DONG17,DONG45 was 346.9 mg/g,331.6 mg/g and 446.5 mg/g,respectively.The three strains possessed better fatty acid compositions and rich essential amino acids,and had a potential nutritional value as aquaculture feeds.%从东极岛海域海泥和海水中分离筛选海洋红酵母,对红酵母进行营养成分分析,旨在探讨红酵母作为水产饵料的营养价值.通过形态观察及ITS序列分析鉴定分离酵母菌株,并进行脂肪酸和氨基酸组成测定,评价其营养成分.结果表明:菌株RI21和DONG17被鉴定为海洋胶红酵母(Rhodotorula mucilaginosa),菌株DONG45被鉴定为红冬孢酵母(Rhodosporidium paludigenum).3株海洋红酵母的脂肪酸主要组分为C16∶0、C18∶0、C18∶1(n-9)和C18∶2(n-6);总氨基酸含量:RI21为346.9 mg/g,DONG17为331.6 mg/g,DONG45为446.5 mg/g.这3株红酵母具有优良的脂肪酸组成、丰富的必需氨基酸,有作为水产养殖饲料的潜在营养价值.