巨噬细胞移动抑制因子过表达对成骨细胞骨形态蛋白-2和血管内皮生长因子表达的影响

摘要

Objective To discuss the overexpression of macrophage migration inhibitory factor (MIF) on bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) in osteoblasts.Methods Human osteoblasts were isolated from 5 patients,and then pcDNA3.0-MIF was constructed and transfected into osteoblasts.The effect of MIF overexpression on BMP-2 and VEGF in osteoblasts was detected.Results MIF overexpression was verified (P ＜0.05).MIF,and BMP-2 and VEGF mRNA and protein levels were higher in transfected cells than in control cells (P ＜ 0.05).Conclusion Overexpression of MIF up-regulated the BMP-2 and VEGF in osteoblasts.%目的 观察巨噬细胞移动抑制因子(MIF)在成骨细胞中的表达及其对成骨细胞骨形态蛋白-2(BMP-2)和血管内皮生长因子(VEGF)表达的影响.方法 提取5例人髂骨成骨细胞,PcDNA3.0构建MIF过表达系统导入成骨细胞中,检测MIF、BMP-2和VEGF的表达.结果 MIF的稳定转染使成骨细胞的MIF过表达(P＜0.05).MIF基因mRNA和蛋白表达在转染组的表达量显著高于对照组中的表达量(P＜0.05)；VEGF和BMP-2的mRNA和蛋白的表达量在转染组也明显增高(P＜0.05).结论 MIF过表达在成骨细胞中上调VEGF和BMP-2的表达.