从自然界中筛选出可分泌高产木聚糖酶的黑曲霉,并对其产酶条件和酶学特性进行优化和分析.结果表明:该菌株培养48 h后,所产的木聚糖酶比酶活最高,达到1645.66 U/g;通过培养基的优化,其比酶活达到了2 698.28 U/g.最优培养基组成(质量分数):麸皮75.0％、玉米12.5％、豆粕12.5％.该酶的最适温度为37℃,最适pH为4.5.%The microbes with high levels of xylanase production were selected. The results showed that As-pergillus niger, xylanase was isolated with the high activity of 1 645. 66 U/g at 48 h cultivatiion (P < 0. 05). Xylanase activity reached 2 698. 28 U/g after medium selection (P < 0. 05). The optimal medium composition (in mass)was wheat bran 75. 0% , corn 12. 5% , and soybean meal 12. 5%. The optimal temperature and pH were 37 ? and 4.5, respectively (P < 0. 05).
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