超声辅助水代法提取芍药籽油工艺条件优化

摘要

研究超声辅助水代法提取芍药籽油的工艺条件,并对其脂肪酸组成进行分析.研究了超声时间、液料比、超声功率和超声温度4个主要因素对芍药籽出油率的影响.在单因素试验的基础上,采用响应面法对超声辅助水代法提取工艺进行了优化.优化后的最佳提取工艺条件为:超声时间56 min,超声温度54℃,液料比8:1,超声功率885 W.在最佳工艺条件下,芍药籽出油率为27.99%.利用GC-MS分析芍药籽油脂肪酸组成,共鉴定出19种脂肪酸,主要脂肪酸为亚麻酸(48.01%)、亚油酸(41.30%),总不饱和脂肪酸含量大于90%.%The process conditions of ultrasound-assisted aqueous extraction of oil from Paeonia lactiflora Pall. seed were studied, and fatty acid composition of the oil was analyzed. The effects of four factors on extraction rate of Paeonia lactiflora Pall. seed oil were studied, including ultrasonic time,ratio of liquid to solid, ultrasonic power and ultrasonic temperature. Based on single factor experiment, the extraction process of Paeonia lactiflora Pall. seed oil was optimized by response surface methodology. The optimal extraction conditions were obtained as follows:extraction time 56 min,extraction temperature 54℃,ratio of liquid to solid 8:1 and ultrasonic power 885 W. Under these conditions,the extraction rate of Paeonia lactiflora Pall. seed oil was 27. 99%. The fatty acid composition of Paeonia lactiflora Pall. seed oil was analyzed by GC-MS,and nineteen kinds of fatty acids were identified. The main fatty acids in Paeonia lactiflora Pall. seed oil were linolenic acid(48. 01%)and linoleic acid(41. 30%),and the total content of unsaturated fatty acids was above 90%.