微波辅助提取核桃壳多糖及其抗氧化活性研究

摘要

采用单因素试验和正交试验对核桃壳多糖的微波辅助提取工艺进行优化,并对其抗氧化活性进行研究.结果表明,微波辅助提取核桃壳多糖的最优工艺条件为:料液比1∶40,微波提取温度70℃,微波提取时间6 min.在最优工艺条件下,核桃壳多糖提取率为2.24％.核桃壳多糖对羟基自由基和超氧阴离子自由基均表现出较好的清除能力,且在一定范围内对二者的清除作用呈现良好的量效关系.%The microwave-assisted extraction process and antioxidant activity of walnut shell polysaccharide were investigated.The results showed that the optimal microwave-assisted extraction conditions were obtained as follows:solid-liquid ratio 1 ∶ 40,extraction temperature 70 ℃ and extraction time 6 min.Under these conditions,the extraction rate of walnut shell polysaccharide was 2.24％.The walnut shell polysaccharide had better scavenging activity on · OH and O2-·,and the scavenging rates showed dose-effect relationship to a certain extent.