脂多糖对类风湿关节炎外周血单核细胞Toll样受体4、髓样分化因子88蛋白表达的影响

摘要

目的：观察脂多糖（LPS）对类风湿关节炎（RA）患者外周血单核细胞 Toll 样受体4（TLR4）、髓样分化因子88（MyD88）蛋白表达的影响。方法采用活动期 RA 患者外周血进行单核细胞的分离培养，并与健康志愿者做对照。分为以下5组观察：健康对照组、健康＋LPS 组、RA 对照组、RA ＋ LPS 组、RA ＋LPS ＋TAK-242组，并采用 Western blot 法检测各组 TLR4、MyD88蛋白的表达。结果健康＋LPS 组或 RA ＋LPS 组 TLR4、MyD88蛋白表达与健康对照组或 RA 对照组比较均明显升高（均 P ＜0．01）；RA 对照组或 RA ＋LPS 组 TLR4、MyD88蛋白表达与健康对照组或健康＋LPS 组比较均明显升高（均 P ＜0．01）；RA ＋LPS ＋TAK-242组 TLR4、MyD88蛋白表达与 RA ＋LPS 组比较明显降低（均 P ＜0．01）。结论RA 活动期单核细胞可能存在 TLR／MyD88信号通路的激活，且对 LPS 的刺激反应性增强。%Objective To observe the effect of lipopolysaccharide(LPS)on expression of TLR4 and MyD88 protein in peripheral blood monocytes cells of rheumatoid arthritis(RA).Methods Peripheral blood monocytes cells of active RA patients were isolated and cul-tured,healthy volunteers as control.Specimen were assignedinto the following five groups:the healthy control group,health + LPS group,RA control group,RA +LPS group,and RA +LPS +TAK-242 group.The expressions of TLR4 and MyD88 protein were de-tected by Western blot.Results The expressions of TLR4,MyD88 protein in health +LPS group or in RA +LPS groupwere signifi-cantly higher than those in healthy control group or in RA control group(all P <0.01).The expressions in RA control group or in RA+LPS group were significantly higher than those in healthy control group or health +LPS group(all P <0.01).The expressions in RA +LPS +TAK-242 group were significantly lower than those in RA +LPS group(all P <0.01).Conclusion Monocytes cells of active RA patients may haveTLR /MyD88 signaling pathway activation and their reactivity to LPS stimulation is increased.