Enabling the Development of Genomics Expertise for Next-Generation Sequencing Novices: An application toward pathogen detection - (PPT)

摘要

Detects all pathogens, including RNA viruses. Detects only viable pathogens, since RNA degrades quickly after cell death. More sensitive than DNA for bacteria. Certain pathogen transcripts can be very abundant and provide improved sensitivity (especially rRNA, though not high resolution). Quantification of gene expression levels for the pathogen (invasive vs. commensal, antibiotic resistance, etc.). Quantification of gene expression levels of the host (RNA biomarkers).