首页> 外文OA文献 >Molecular cloning of a Pseudomonas paucimobilis gene encoding a 17-kilodalton polypeptide that eliminates HCl molecules from gamma-hexachlorocyclohexane.
【2h】

Molecular cloning of a Pseudomonas paucimobilis gene encoding a 17-kilodalton polypeptide that eliminates HCl molecules from gamma-hexachlorocyclohexane.

机译:编码17-千达尔顿多肽的假单胞菌假单胞菌基因的分子克隆,该多肽可从γ-六氯环己烷中消除HCl分子。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Pseudomonas paucimobilis UT26 is capable of growing on gamma-hexachlorocyclohexane (gamma-HCH). A genomic library of P. paucimobilis UT26 was constructed in Pseudomonas putida by using the broad-host-range cosmid vector pKS13. After 2,300 clones were screened by gas chromatography, 3 clones showing gamma-HCH degradation were detected. A 5-kb fragment from one of the cosmid clones was subcloned into pUC118, and subsequent deletion and gas chromatography-mass spectrometry analyses revealed that a fragment of ca. 500 bp was responsible for the conversion of gamma-HCH to 1,2,4-trichlorobenzene via gamma-pentachlorocyclohexene. Nucleotide sequence analysis revealed an open reading frame (linA) of 465 bp within the fragment. The nucleotide sequence of the linA gene and the deduced amino acid sequence showed no similarity to any known sequences. The product of the linA gene was 16.5 kDa according to sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
机译:假单胞菌UT26能够在γ-六氯环己烷(γ-HCH)上生长。通过使用宽宿主范围的粘粒载体pKS13,在恶臭假单胞菌中构建了P. paucimobilis UT26的基因组文库。通过气相色谱法筛选2,300个克隆后,检测到3个显示γ-六氯环己烷降解的克隆。来自粘粒克隆之一的5-kb片段被亚克隆到pUC118中,随后的缺失和气相色谱-质谱分析表明,该片段大约为1。 500 bp负责将γ-六氯环己烷经γ-五氯环己烯转化为1,2,4-三氯苯。核苷酸序列分析揭示了该片段内465 bp的开放阅读框(linA)。 linA基因的核苷酸序列和推导的氨基酸序列与任何已知序列均无相似性。根据十二烷基硫酸钠-聚丙烯酰胺凝胶电泳,linA基因的产物为16.5 kDa。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号