porcine circovirus infectious chimeric nucleic acid molecule (pcv-12), viral vector or plasmid containing said molecule, suitable host cell, avirulent infectious chimeric porcine circovirus, process for producing immunogenic polypeptide product, product thus obtained, viral vaccine, process for preparation of said infectious pcv2 molecular DNA molecule, clone, plasmid or biologically functional viral vector containing said clone, infectious reciprocal chimeric pcv2-1 nucleic acid molecule, as well as use of said clones

摘要

"chimeric infectious DNA clones, chimeric porcine circovirus and uses thereof". The present invention relates to infectious DNA clones, porcine circovirus (pcv) chimeric DNA clones, vaccines and means of protecting pigs against viral infection or post weaning multisystemic wear syndrome (pmws). pcv2. The new chimeric infectious DNA clone and its derivative, a virulent chimeric virus are constructed from the nonpathogenic pcv1 wherein the pathogenic pcv2 orf immunogenic gene replaces a nonpathogenic pcv1 gene, preferably in the same position. The chimeric virus advantageously maintains the nonpathogenic pcv1 phenotype but activates specific immune responses against the pathogenic pcv2. The invention further encompasses immunogenic polypeptide expression products.