Evaluation of DNA preparation methods combined with different PCR-based assays for Coxiella burnetii detection in milk

摘要

Introduction - Coxiella burnetii (Cb) is the causative agent of Q fever, a zoonosis that occurs worldwide. Due to health concerns, unpasteurized cow's milk and a number of dairy products produced by unpasteurized milk may contain virulent Cb. PCR method is commonly employed for sensitive, specific and rapid test for Cb detection in biological samples including milk.