首页> 外国专利> PNA Pepetide nucleic acid probe for identifying Coxiella burnetii and Method for identifying Coxiella burnetii using the same

PNA Pepetide nucleic acid probe for identifying Coxiella burnetii and Method for identifying Coxiella burnetii using the same

机译:用于鉴定伯氏柯氏杆菌的pna肽核酸探针和使用该探针的鉴定伯氏柯氏杆菌的方法

摘要

The present invention relates to a peptide nucleic acid (PNA) probe for detecting Coxiella burnetii and a Coxiella burnetii detection method using the same. More specifically, according to the present invention, a Coxiella burnetii gene is amplified by using a PNA probe represented as a base sequence selected from groups consisting of sequence numbers 4 to 6, and a primer represented as the sequence numbers 1 and 3. The amplified gene is hybridized with the PNA probe, and Coxiella burnetii is detected through a melting curve analysis of the hybridized material. Accordingly, the PNA probe can detect Coxiella burnetii, which is a cause bacillus of Q-fever, at higher sensitivity and correctness than that of a conventional polymerase chain reaction (PCR) method, and can easily discriminate false positiveness, thereby being useful for Q-fever diagnosis.
机译:本发明涉及用于检测伯氏柯氏杆菌的肽核酸(PNA)探针和使用该探针的方法。更具体地,根据本发明,通过使用表示为碱基序列的PNA探针扩增伯氏柯氏杆菌基因,所述碱基序列选自由序列号4至6组成的组,以及由引物表示为序列号1和3。该基因与PNA探针杂交,并通过杂交材料的熔解曲线分析检测到了柯氏杆菌。因此,与传统的聚合酶链反应(PCR)方法相比,PNA探针可以以更高的灵敏度和正确性检测作为Q热的致病杆菌的柯氏杆菌。发烧诊断。

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