Quantification of 2-Acetyl-l-pyrroline from the Aroma Rice Germplasm by Gas Chromatography

摘要

This study was conducted to optimize the analysis condition and quantity the 2-acetyl-1-pyrroline (2AP) in the brown aroma rice. Extraction effect of the solvent for 2AP was the order of ethanol>acetonitrile>methanol in the range from 30 to 90°C. Inthe extraction time of 15, 30, 60, and 90 min, the 30 min had the highest 2AP concentration, and it was decreased according to lapse of time. At grinding time, 5 sec resulted in highest 2AP concentration. It was recommended that five sec grinding time, using ethanol, at 90°C for 30 min was the optimization conditions to quantify the 2AP. Hyangmibyeo2ho and Aranghyangchalbyeo were mild aroma. In the foreign aroma rice, 11 of 19 accessions of Indica types and 2 of 6 accessions of Japonica types were morethan mild aroma. Finally, 30 accessions of aroma rice were selected based on their 2AP concentration and agronomic traits.