A novel loop-mediated isothermal amplification method for detection of the carrot materials in foods

摘要

Carrot is an important elicitor of food allergy. In this study, we developed a novel molecular detection assay based on loop-mediated isothermal amplification technology for the detection of carrot in food materials. The assay had sensitivity up to 4 pg of the purified carrot DNA and had no cross-reactions with other species, such as fennel, parsley, anise, caraway, cumin, walnut, pecan, peanut, cashew, almond, pistachio, sunflower seed, sesame seed, chestnut, soy, barley, wheat, oat, rye, rice, cabbage, green pepper, onion, celery, cucumber, beef, mutton, pork, chicken and shrimp. The validation study demonstrated high reproducibility and specificity. This assay was proved to be a potential tool for the detection and label management of carrot allergens in foods.