A rapid and sensitive chemiluminescence enzyme-linked immunosorbent assay for the determination of fumonisin B-1 in food samples

摘要

An enzyme-linked immunosorbent assay (ELISA) based on polyclonal antibody with enhanced chemiluminescent (ECL) detection of fumonisin B, (FBI) in food samples has been developed. Assay conditions, including concentrations of antibody and enzyme conjugate, competition time and so on, were optimized. The effects of pH and two different organic solvents were investigated. The optimized ECL-ELISA system allowed FBI determination in a linear working range of 0.14-0.9 mu g L-1 with IC50 value of 0.32 mu g L-1 and a limit of detection of 0.09 mu g L-1. The ECL-ELISA was about 10 times more sensitive and about 30% time less than that of colorimetric ELISA using the same antibody and HRP-conjugate. Good recoveries with spiked food samples were obtained, and the results correlated well with those obtained using conventional direct competition ELISA assay and HPLC method, which indicated that ECL-ELISA was capable of being applied for the specific detection and routine monitoring of FBI in food samples. (c) 2006 Elsevier B.V. All rights reserved.