Rapid Determination of Fumonisin B_1 in Food Samples by Enzyme-Linked Immunosorbent Assay and Colloidal Gold Immunoassay

摘要

A rapid enzyme-linked immunosorbent assay(ELISA)test(microwell plate)and a membrane-based colloidal gold immunoassay in flow-through and lateral-flow formats for the rapid detection of fumonisin B_1(FB_1)were developed.The rapid microwell assay can be completed within 20 min with the detection limit of 0.5 +-0.2 mug/L.Membrane-based colloidal gold immunoassays had a visual detection limit of 1.0 mug/L for FB_1 with the detection time of <10 min.Matrix interference was eliminated by 15-fold dilutions of methanol extracts with buffer.These immunoassays can be used as quantitative or qualitative tools for the rapid detection of FB_1 residues in 10-20 min on-site.