Development of a colloidal gold-based immunochromatographic assay for the rapid detection of aflatoxin B_1 in food samples

摘要

A rapid and sensitive competitive immunochromatographic assay for aflatoxin B_1 (AFB_1) based on colloidal gold-antibody probe was developed. The limit of detection for AFB1 standard solution was 0.5ng/ml and for foodstuff sample was 10μg/Kg. Other aflatoxins including AFM_1, AFB_2, AFG_1, and AFG_2 have less cross-reactivity to the strip. The storge life of the strip was at least 12 monthes. The method was well agreement with the ELISA method by testing food samples. This method is suitable for rapid testing of AFB_1 on site.