以抗原-抗体的免疫反应及化学发光为基础,以辣根过氧化物酶(HRP)标记伏马菌素B1(FB1)为半抗原、鲁米诺-H2 O2为发光底物,对羟基联苯为化学发光增强剂,建立了一种新型的FB1快速检测方法——增强化学发光酶联免疫分析法,相关系数R为0.9916,线性范围为200~1400μg/L,检测限为91.3μg/L.在不同的加标水平下,回收率为85.9%~115.4%,与之相对应的板内RSD为9.1%~10.2%(n=6),板间RSD为10.6%~12.4%(n=6),与其他真菌毒素交叉反应率较低.与传统的检测方法(酶联免疫吸附测定和高效液相色谱法)进行了比较,结果表明,本研究建立的方法灵敏度高、特异性好、操作简单、检测快速,能够用于大批量实际样品的快速检测.%Based on the immune reaction between antigen and antibody and chemiluminescence , a new method for rapid detection of fumonisin B 1 was established using luminol-H2 O2 as luminescent substrates and the hydroxy diphenyl as chemiluminescence enhancer , and named as enhanced chemiluminescence enzyme-linked immunoassay(ECLEIA).On the basis of optimizing the experimental conditions , the limit of detection was 91.3 μg/L, and the linear range of the ECLEIA method for FB 1 determination was from 200 μg/L to 1400μg/L, while the correlation coefficient was 0.9916.Furthermore, the relative stand-ard deviations of intra-assay and inter-assay were 9.1%~10.2%( n=6 ) and 10.6%~12.4% ( n=6),respectively.The recoveries were 85.9%~115.4%, and the cross-reactivity rates with object ana-logues were low .Compared with other common detection methods ( HPLC and ELISA ) , ECLEIA method had high sensitivity , high specificity , sample operation and rapid detection , which could be used for the fast testing of large quantities of real samples .
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