BLACK RICE ANTHOCYANINS AMELIORATE RETINAL PHOTOCHEMICAL DAMAGE IN RATVIA ANTI-OXIDATION AND ANTI-APOPTOSIS

摘要

It was established that black rice anthocyanins (BRACs) provided potent anti-oxidative and anti-apoptotic effects wherever in vitro or in vivo. In this study, the protective effects of BRACs against retinal photochemical damage and the mechanisms that might be associated were investigated in Sprague-Dawley rats. Sixty rats were assigned randomly to two groups, which were treated with (n=30) or without BRACs (n=30) by intragastric administration for 15d respectively. Then both groups were exposed to 3000 +- 200 lux fluorescent light for 0~24h. After treatments, the cytoprotective role and antioxdant effects of BRACs were assessed in retinas. Our result showed that the light exposure resulted in severely functional and structural damage in retinas. And supplementary of BRACs effectively prevented injure as previously stated and maintained its normal junction and structure. Furthermore, supplementary BRACs markedly reduced the photoreceptor cells apoptosis induced by light stress (P< 0.05), and also decreased malondialdehyde level (P< 0.05) and elevated the activities ofsuperoxide dismutase (SOD), glutathione S-transferase (GST) and glutathione peroxidase (GSH-PX) in retinas (P<0.05). In conclusion, these result suggested that supplementation with BRACs can effectively protect the retina against photochemical damage in rats, which may be due in mostly to its potent antioxidant and anti-apoptotic capacity.