A process for the aqueous enzymatic extraction of corn oil from dry milled corn germ and enzymatic wet milled corn germ (e-germ).

摘要

A bench-scale aqueous enzymatic method was developed to extract corn oil from corn germ from either a commercial corn dry mill or corn germ from a newly-developed experimental enzymatic wet milling process (E-Germ). With both types of germs, no oil was extracted when acidic cellulase was the only enzyme used. Pre-treating dry milled corn germ by heating it in boiling water or microwave pretreatment, followed by enzymatic extraction with the acidic cellulase resulted in oil yields of about 43% and 57%, respectively. A two-step process, combining both acidic cellulase and alkaline protease treatments, with no heat pretreatment, achieved oil yields of 50-65% from dry milled corn germ and 80-90% from E-Germ. (c)US Government 2009.