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Bacteriocin inhibition of two glucose transport systems in Listeriamonocytogenes

机译:细菌素抑制李斯特菌中两个葡萄糖转运系统

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Listeria monocytogenes transports glucose by proton motive force-mediated and phosphoenolpyruvate-dependent phosphotransferase systems (PEP-dependent PTS). Inhibition of both systems by nisin, pediocin JD and leuconosin S is reported here for four strains of L. monocytogenes. Intracellular and extracellular adenosine triphosphate (ATP) and extracellular inorganic phosphate were measured in energized L. monocytogenes Scott A cells to determine whether inhibition of the PEP-dependent PTS might occur as a result of bacteriocin-induced leakage,of intracellular components. Addition of nisin resulted in a decrease in intracellular ATP with an increase in extracellular ATP. Leuconosin S and pediocin JD induced a depletion of intracellular ATP. ATP efflux was low for the leuconosin S-treated cells and barely detectable for pediocin JD-treated cells. Addition of nisin, leuconosin S and pediocin JD induced efflux of inorganic phosphate. It appears that bacteriocin-mediated inhibition of the glucose PEP-dependent PTS occurs as a result of hydrolysis or efflux of ATP, PEP and other essential molecules from L. monocytogenes cells.
机译:单核细胞增生李斯特菌通过质子动力介导的和磷酸烯醇丙酮酸依赖性磷酸转移酶系统(PEP依赖性PTS)转运葡萄糖。此处报道了乳酸链球菌肽,pediocin JD和leuconosin S对这两种系统的抑制作用,涉及四种单核细胞增生李斯特菌。在通电的单核细胞增生李斯特氏菌Scott A细胞中测量细胞内和细胞外三磷酸腺苷(ATP)和细胞外无机磷酸,以确定是否由于细菌素诱导的细胞内成分泄漏而抑制PEP依赖性PTS。乳酸链球菌肽的添加导致细胞内ATP的减少而细胞外ATP的增加。 Leuconosin S和pediocin JD诱导细胞内ATP耗竭。对于经亮骨球蛋白S处理的细胞,ATP外排较低,而对于经pediocin JD处理的细胞则几乎检测不到。乳酸链球菌肽,leuconosin S和pediocin JD的添加引起无机磷酸盐的外流。看来细菌素介导的对葡萄糖PEP依赖性PTS的抑制是由于单核细胞增生李斯特氏菌细胞中ATP,PEP和其他必需分子的水解或外排而发生的。

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