Optimization ultrasound-assisted extraction of anthocyanins from cranberry using response surface methodology coupled with genetic algorithm and identification anthocyanins with HPLC-MS_2

摘要

Cranberry contains abundant anthocyanins. In this study, the extraction process for theultrasound-assistedextraction (UAE) of anthocyanins from cranberry was optimizedusing response surface methodology coupled with genetic algorithm. The optimum extractionparameters to achieve the highest yield of anthocyanins (7.25 ± 0.02) mg/g fromcranberry via UAE were obtained at an extraction temperature of 53°C, ultrasoundpower of 310 W, ultrasound time of 33 min, and ethanol concentration of 52%. High-speedcountercurrent chromatography was used to separate and enrich main anthocyaninsfrom cranberry. The purified anthocyanin components were analyzed by HPLC-MS2.Seven major kinds of anthocyanins were detected and identified for the first timepeonidin-3-(6-malon)-glucoside with a purity of 82.13% and pelargonidin-3-(6-malon)-glucoside with a purity of 85.37% in cranberry. Hence, ultrasound-assisted extraction ofanthocyanins was an effective and rapid method. This method has a broad applicationprospect in the extraction of bioactive substances from natural plant resources.