食品中の甘味料分析における迅速な改良透析法の開発(第2報)

摘要

食品に添加された甘味料(サッカリンナトリウム(Sa),アセスルファムカリウム(AK),アスパルテーム(APM),ズルチン(Du))の抽出および精製を行う迅速な透析法を開発した.従来法では48時間の透析が必要な穀物調製品のクッキーに,0.02g/kgの甘味料を添加して検討を行った.透析は透析総量を200mLから100mLに減らし,透析膜の有効長を55cmから50cmに変更した.さらに透析液に30％メタノールを用い,50℃に加温し,160rpmで振とうして行った.その結果,透析1時間後のSa,AK,APMおよびDuの回収率(％)は,それぞれ99.3,102.3,103.8,99.4と良好であった.%a rapid dialysis method was developed for the extraction and purification of four artificial sweeteners, namely, sodium saccharide (Sa), acesulfame potassium (AK), aspartame (APM), and dulcin (Du), which are present in various foods.The method wasevaluated by the addition of 0.02 g/kg of these sweeteners to a cookie sample, in the same manner as in the previous report. Revisions from the previous method were: reduction of the total dialysis volume from 200 to 100 mL, change of tube length from 55 to 50 cm, change of dialysate from0.01 mol/L hydrochloric aqueous solution containing 10% sodium chloride to 30% methanol solution, and change of dialysis conditions from ambient temperature with occasional shaking to 50℃ with shaking at 160 rpm. As a result of these revisions, the recovery reached 99.3-103.8% with onehour dialysis. The obtained recovery yields were comparable to the recovery yields in the previous method with four hour dialysis.