Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens

摘要

The present study evaluate the antibiotic potential of crude culture extract of lactic acid bacteria on multi-drugs resistance farm animal pathogen. Eight (8) different pathogens associated with Farm animals were used in this study. The pathogens were collected from different part of the body of different animal which include, Vagina of cow, Stool of cow; Male cow anus; Vagina of sheep; Vagina of goat, Male Goat Anus, Penis of goat and Female goat anus. The Confirmation of Pathogens, Isolation of crude extract of Lactic acid bacteria, Cell Free Supernatant and determination of antibacterial activity was done using standard method. The results show that Streptococcus agalactiae VIS, Staphylococcus aureus STC and Escherichia coli VRC were resistance to 4 out of 13 antibiotics. While Staphylococcus aureus FGA, and Escherichia coli (PEG, MGA and VRG) were resistances to 3 out of 13 antibiotic tested. Staphylococcus aureus MCA is sensitive to all antibiotics tested. Clear zone of inhibition was observed in both the Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria which ranged from 7mm to 25mm. The results further revealed that both Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria can be used in the in the treatment of multidrug resistance of pathogens. The isolation process of Crude Culture Extract (CCE) and it uses as an antibacterial agent is a simple process that do not required the use of any sophisticated equipment and techniques, thus it may be use in the treatment of multidrug resistance pathogens in both local and modern animal farms.