Antioxidant Activities and the In Vitro Effects on K562 Cell Viability ofFlavonoids Obtained from Onionskin by Different Extraction Methods

摘要

In order to effectively extract specific flavonoids with high biological activity from onionskin, NaNO2-Al(NO3)3-NaOH coloration method, spectrophotometry and MTT method were adopted to study the yield rate and contentof flavonoids obtained from alcohol water and alkali water extraction processes, and the antioxidant activities and the invitro effects on K562 cell viability. The results indicate that yield rates of flavonoids obtained by alcohol water and alkaliwater extraction methods was 3.76% and 2.37% respectively, solid flavonoids contents were 42.20% and 76.21%; underthe same concentration of total flavonoids, alkali water extract has weaker effect in removing superoxide anions andDPPH ? radicals, they have similar effect in removing nitrite and hydroxyl free radicals; in the test concentration range (40~ 200 μg / mL), they have similar effect in inhibiting the proliferation activity of K562 cells, the maximum inhibition rateof flavonoids obtained by alkali water extraction method is 77.71%, while maximum inhibition rate of flavonoid obtainedby alcohol water extraction method is 70.64%. Both the flavonoids obtained from onionskins by alcohol water extractionmethod and alkali water extraction method have good biological activity. The alkali water extraction method is simplewithout any alcohol consumption, and the product purity is high, although the yield rate and certain biological activity offlavonoids obtained by alkali water extraction method are lower than those obtained by alcohol water extraction method,it still has certain significance in industrial production.