Effect of miR-301a/PTEN pathway on the proliferation andapoptosis of cervical cancer

摘要

The aim of this study was to evaluate the effect of the miR-301a/PTENpathway in cervical cancer. miR-301a and PTEN expression were measuredby quantitative real-time PCR (qRT-PCR) in tissues samples and HeLacells. PTEN protein level was determined by Western blotting. Dualreporter luciferase assay was performed to validate as a direct target of miR-301a. The gain-and loss-of function assay was performed by miR-301a overexpressionand silencing. Cell proliferation was monitored by cell counting Kit-8(CCK-8). Cell apoptosis was quantitated by flow cytometry. SPSS wasused to analyze the significant difference in the treatments. miR-301ademonstrated a significantly higher expression in cervical carcinomatissues compared with the paired non-carcinoma tissues(  = 12), while PTEN expression was found to besignificantly lower in cervical carcinoma tissues than their pairednon-carcinoma tissues (  = 12). In addition, PTEN wasidentified as the direct target of miR-301a. Moreover, overexpressionof miR-301a significantly promoted HeLa cells proliferation andanti-apoptosis which had a reverse pattern after PTEN overexpression.Our results confirm as a direct target ofmiR-301a in HeLa cells and suggest that miR-301a/PTEN pathwaycontributes to the development and progression of cervical cancer.