Anti-Cancer Effects of miR-181/PTEN/PI3K/Akt Signaling Pathway Suppressed Cell Proliferation and Migration of Human Cervical Cancer

摘要

Cervical cancer ranks the second in the incidence of common women malignant tumors, which is the commonest malignant tumor in women's reproductive organs. In present research, we evaluated the possible antitumor effect of miR-181 on cervical cancer and explored the molecular mechanism of this phenomenon. Briefly, MTT, LDH activity kit and flow cytometry were used to detect cell growth, LDH activity and cell apoptosis rate of HeLa cells. The experiment correlation protein expression levels of PTEN, phosphatidylinositol-3-hydroxykinase (PI3K) and phosphorylation (p)-Akt (protein kinase B) were measured by Western blot analysis. In present research, this research observed that miR-181 weakened the cell proliferation activity of cervical cancer cells and induced their caspase3/9 to promotion of cell apoptosis. PTEN expression was induced at the genetic and protein levels, and the PI3K/Akt expressions were inhibited at the protein levels in cervical cancer cells. SiPTEN weakened the antitumor effect of miR-181 on cervical cancer by activation of PI3K/Akt signaling pathway.