茶多酚对卵巢癌SKOV3细胞自噬水平的影响及相关机制研究

摘要

Objective To investigate the effect of EGCG-induced autophagy on human ovarian cancer cell line SKOV3 and to explore the mechanism. Methods Human ovarian cancer cell SKOV3 were treated with EGCG, then autophage-associated protein LC3-II and AKT protein expression on the cells was detected by Western blot. Results After EGCG treatment, the level of LC3-II was up-regulated in a dose dependent manner, while the level of phospho-AKT was decreased. Pretreatment with AKT activator insulin-like growth factor 1, the EGCG-induced LC3-II expression was suppressed. Conclusion EGCG can induce autophagy in huaman ovarian cancer cell SKOV3 through AKT signaling pathway.%目的：研究茶多酚（EGCG）对人卵巢癌SKOV3细胞自噬水平的影响及相关机制。方法用EGCG处理SKOV3细胞，Western blot检测自噬相关蛋白LC3-II及蛋白激酶B（PKB）信号通路相应蛋白表达变化。结果 EGCG处理SKOV3细胞后，自噬相关蛋白LC3-II表达上调，并呈一定的时间浓度依赖性。EGCG处理SKOV3细胞后，AKT的磷酸化水平下调，AKT激活剂胰岛素样生长因子1（IGF-1）预处理后，自噬相关蛋白LC3-II高表达被抑制。结论 EGCG通过AKT介导的信号通路诱导SKOV3细胞自噬水平升高。