鹿茸多肽对异丙肾上腺素诱发大鼠心肌损伤的保护作用

摘要

目的:观察鹿茸多肽对异丙肾上腺素(isoproteronol,ISO)诱发大鼠心肌缺血损伤的保护作用.方法:皮下注射ISO建立大鼠急性心肌缺血模型.检测注射鹿茸多肽后心肌缺血大鼠血清肌酸激酶(CK)、乳酸脱氢酶(LDH),天门冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)、丙二醛(MDA),以及心肌组织SOD、MDA,心肌梗死面积、心电图ST段.结果:鹿茸多肽能明显减少ISO诱发心肌缺血损伤大鼠心肌梗死面积,抑制心电图ST段的升高,降低血清CK、LDH、AST活性、MDA含量以及心肌组织MDA含量,升高血清及心肌组织SOD活性.结论:鹿茸多肽对ISO诱发的大鼠心肌缺血损伤具有保护作用,其机制可能与抗氧化作用有关.%Objective:To investigate the cardioprotective effects of the peptides from the velvet antler (VAP) on acute ischemic myocardial injury induced by isoproterenol in rats.Method:The model rats of myocardial ischemia injury produced by subcutaneous injection(sc) of ISO was used to observe the effects of VAP on the ischemic size,ST elevation,serum levels of creatine kinase (CK),lactate dehydrogenase (LDH),aspartate transaminase (AST),superoxide dismutase (SOD),malondialdehyde (MDA),and levels of SOD,MDA in myocardial tissue.Results:VAP could significantly decrease ST elevation,infarct size,serum levels of CK,LDH,AST,MDA and myocardial MDA,increase serum and myocardial SOD activity in the rats of acute ischemic myocardial injury induced by isoproterenol.Conclusions:VAP exerts significant cardioprotective effects against acute ischemic myocardial injury induced by isoproterenol in rats,likely through its antioxidant and anti-lipid peroxidation properties.