Biodegradable gelatin/β-tricalcium phosphate composite microspheres as localized delivery of growth factor- Velvet Antler Polypeptides by water-in-oil (W/O) emulsion process

摘要

This work introduce a new conventional method to prepare ternary composite moicrospheres nano β-tricalcium phosphate/ gelatin/ Velvet Antler Polypeptides by water-in-oil (W/O) emulsion process. The nano β-tricalcium phosphate (TCP) and Velvet Antler Polypeptides (VAP) are embedded into the gelatin spheres. SEM image indicates the composite spheres have a homogeneous microstructure, a perfect monodispersity and also a uniform size of about 45um. The optical microscopy picture shows the nano TCP granules are embedded inside the composite. From the SELDI-TOF-MS spectrum, it can be seen that the VAP is brought into the inside of the spheres by the same emulsion process. In the reaction, the growth factor through ion complex binds to the gelatin matrix by the different isoelectric point (IEP) of gelatin (8.5) and VAP (4.5). DSC-TGA image illustrates the 90% TCP is embedded inside the gelatin spheres through the emulsion process and strong interaction appeared between TCP and gelatin that come from the calcium ions from the TCP and the carboxyl ions from gelatin.