响应面法优化深层发酵樟芝总三萜的提取工艺

摘要

采用响应面法对樟芝深层培养中总三萜的提取工艺进行优化.根据Box-Benhnken的中心组合实验设计原理,在单因素实验的基础上,选取溶剂浓度、提取温度和液固比为变量,应用响应面法进行三因素三水平的实验设计.以总三萜得率作为响应值,建立了樟芝总三萜提取的回归模型,对其提取条件进行进一步优化.结果表明,优化的总三萜提取条件为乙醇浓度86％,提取温度75 ℃,液固比37.进一步的实验也验证了该模型的有效性.%The optimum conditions for the extraction of total triterpenoids from submergedly cultured Antrodia camphorata were determined by using response surface methodology ( RSM).A three-level Box-Behnken factonal design was used to investigate the effects of three independent variables,namely solvent composition,extraction temperature and liquid-solid ratio on the response,the yield of total triterpenoida.The independent variables were coded at three levels and their actual values were selected on the basis of preliminary experimental results.Results showed that the generated regression models adequately explained the data variation and significantly represented the actual relationship between the independent variables and responses.A solvent composition of 86％,an extraction temperature of 75 ℃ and a liquid-solid ratio of 37 were found to be optimal for triterpenoid extraction from A.camphorata in submerged culture.By means of additional experiments,the adequacy of this model was conformed.<英文关键词>=Key words:Antrodia camphorata ; submerged culture ; triterpenoid ; response surface methodology ; optimization of extraction