首页> 中文期刊> 《天然产物研究与开发》 >高压脉冲电场法快速提取北五味子中五味子醇甲的工艺研究

高压脉冲电场法快速提取北五味子中五味子醇甲的工艺研究

         

摘要

本文采用正交实验优化提取方法,HPLC法测定五味子醇甲含量,对五味子的快速提取工艺方法进行了研究.结果表明:以80%乙醇作为提取液,电场强度10 kv/cm,脉冲数为10,料液比1∶10为最佳提取工艺;影响因素的大小依次为:电场强度、料液比、脉冲数.此法提取五味子中的五味子醇甲,提取率可达0.76%,得膏率达27.886%.本提取工艺可行,经济、省时、回收率高.%The rapid extracting technology for schisandrol A from Schisandra chinensis was investigated by using orthogonal experimental design,and the content of schisandrol A was detected by HPLC. The optimum extracting technology was obtained as follows; 80% ethanol as for the extract liquid, 10 kv/cm electric field intensity,impulsion frequency of 10, and 1:10 as the ratio for material and liquid. The effect levels of these factors successively were electric field intensity, the ratio for material and liquid,and impulsion frequency. The content of schisandrol A was 0.76% in S. Chinensis,and the yield of extract was 27. 886%. The extracting technology is feasible,economic with high recovery ratio and saving time in the process.

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