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现代中药研究与实践

现代中药研究与实践

CSTPCD

CHSSCD

Research and Practice on Chinese Medicines
本刊是一份以普及为主，提高为辅的中药学术期刊。由安徽省中医管理局主管全国中医药职业技术教育学会、皖南职业学院主办。本刊专门刊登用于指导基层中药工作的新经验、新技术、新方法，具有新颖、实用、通俗的特点。可供各级医院药剂科、中药房、药材公司、医药院校、药检所以及中医药科研部门的工作人员参考
  • 发文量：3761
  • 被引量：1216
  • H指数：1
  • 开始收录时间：1992,6(1)
  • CNKI综合因子：0.69
  • 维普期刊影响因子： 1.27
  • 万方期刊影响因子：0.68
  • 创刊时间：1987
  • 国内刊号/CN：34-1267/R
    国际刊号/ISSN：1673-6427
  • 发行周期：双月刊
    邮发代号：26-85
  • 主管单位：安徽省教育厅
    主办单位：安徽中医药高等专科学校；
现代中药研究与实践-联系信息
  • 主编：叶文才;姚应水
  • 电话：0553-4836136
  • 邮箱：jzzy@chinajournal.net.cn
  • 地址：安徽省芜湖市乌霞山西路18号
  • 邮编：241002
现代中药研究与实践-获奖情况
  • • 获1998年安徽省优秀期刊
  • 收录汇总
  • 刊内文献检索
  • 投稿信息

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  • 摘要：目的 探讨提高盾叶薯蓣经济产量的科学施肥技术.方法 用氯磷钾作底肥,设置不同配比及配比量,分析不同处理间的产量差异.结果 施磷钾肥的产量最高,显著或极显著的高于其他配比;氮钾肥配、氮磷配比和单施氮肥的产量最低.结论 施用适量磷钾肥作底肥,能显著提高产量,种植薯蓣不宜施用尿素作底肥,可将尿素作为追肥施用.
  • 摘要：Objective To investigate the anti-tumor effects of HCPT on the mice models of H22.Methods The mice models of H22 is the experimental observation of the object.Observing inhibitory effect of HCPT on the tumor, weight changes, growth status before and after chemotherapy.Results Positive drug group and HCPT high, medium and low-dose chemotherapy group all inhibit the mice models of H22 (P＜0.05),inhibition rate are 66.12％, 60.26％, 50.16％, 30.94％, respectively.To compare with the model group, the mice living conditions (weight, reaction ability, and so on) have improved in the HCPT chemotherapy group.HCPT medium-dose group has protective effect on the liver and kidney function in mice;while HCPT high-dose group has irreversible damage to the liver and kidney function in mice.Conclusion High, medium and low doses of HCPT have different levels of inhibition on the H22 tumor models growth.%目的 考察羟基喜树碱(HCPT)对小鼠H22模型的抗肿瘤作用.方法 以小鼠H22肝癌模型为实验观察对象.观察HCPT对肿瘤的抑制效果、化疗前后的体重、食物摄取量及生长状况,同时观察HCPT对H22肝癌模型小鼠肝肾功能的保护作用.结果 阳性药对照组及HCPT高、中、低剂量化疗组对小鼠H22肝癌模型均有抑制作用(P<0.05),抑瘤率分别为66.12%、60.26%、50.16%、30.94%.HCPT化疗组与肿瘤对照组相比,小鼠的生活状态(体重、反应能力等)均有一定的改善.HCPT中剂量化疗组对小鼠的肝肾功能有一定的保护作用,而高剂量化疗组对小鼠的肝肾功能有不可逆的损伤.结论 高、中、低剂量的HCPT对H22肝癌模型小鼠的肿瘤生长有不同程度的抑制作用.
  • 摘要：Objective To study the processing method of Hippocampus preparation. Methods To extract Hippocampus by different solvent. Determine total phospholipid content of the extract and total nitrogen content and ratio of dry extraction of the extract. Hydrolysis Hippocampus by four kinds of enzymes, respectively.Results The extraction process with water and ethanol successively can guarantee the ratio of extraction of effective components. Hydrolyzing the residue of Hippocampus by animal protease can maximize the utilization ratio. Conclusion Extracting Hippocampus by solvent and hydrolyzing the residue of Hippocampus by animal protease could offer a processing reference of Hippocampus preparation.%目的 研究海马的制剂加工处理方法.方法 用四种不同的溶剂提取海马,测定提取物总磷脂含量,总氮含量及干膏率;并比较四种不同的酶水解海马的提取效果.结果 用水醇两种溶剂提取海马可保证有效成分的得率,药渣用动物蛋白酶水解可使药材最大限度地得到利用.结论 采用溶剂提取,药渣用酶水解可作为海马制剂加工的一种参考.
  • 摘要：Objective To develop an HPLC method for the quantitative determination of glyeyrrhizic acid in Fuzi Lizhong pills. Methods The analysis was carried out on a Waters Symmetry C18 (4.6 mm×250 mm,5 μm) column with the mixture of methyl cyanides-phosphoric acid (0.017 mol/L, 40: 60) as the mobile phase at a flow rate of 1.0 mL·min-1, the detection wavelength was set at 250 nm and the column temperature was maintained at 35 ℃. Results The calibration curve showed a good linearity with the range of 30.9～154.4 μg·mL-1 for glyeyrrhizie acid, and the mean recovery (n=6) was 98.63％ (RSD=0.99％). Conclusion This method is simple and accurate. And it can be used for the determination of glycyrrhizic acid in Fuzi Lizhong pills.%目的 建立附子理中丸中甘草酸的高效液相色谱分析方法.方法 Waters Symmetry C<,18>(4.6mm×250 mm,5 μm),流动相为乙腈-磷酸溶液(0.017 mol/L,40:60),流速1.0 mL·mm<'-1>,检测波长250nm,柱温35℃.结果 甘草酸在30.9～154.4 μg·mL<'-1>范围内具有良好的线性关系,平均回收率(n=6)为98.63%(RSD=0.99%).结论 该方法快速、准确,可用于附子理中丸中甘草酸的含量测定.
  • 摘要：Objective To establish an HPLC method for the determination of tetrandrine and fangchinoline in Xixianfengshi tablets. Methods The method was performed on C18(4.6 mm× 150 mm, 5 μm) column with acetonitrile-water-triethylamin(70: 30: 0. l) as mobile phase. Flow rate was 1.0 mL/min, and the wavelength was 280 nm. Results The linear ranges of tetrandrine and fangchinoline were 0.344 μg～l.72 μg (r=0.999 9 ) ,0.153 μg～0.765 μg ( r=0.999 9 ), respectively. The average recovery were 99.20％ and 99.26％, respectively,Conclusion This method is proved to be simple, accurate and can be used for quality control of Xixianfengshi tablets.%目的 建立豨莶风湿片中粉防己碱及防己诺林碱的HPLC测定方法.方法 色谱柱为C<,18>柱(4.6mm×l50 mm,5 μm);流动相为乙腈-水-三乙胺(70:30:0.01),流速为1.0 mL/min,检测波长为280nm.结果 粉防己碱及防已诺林碱的进样量分别在0.344 μg～1.72 μg(r=0.999 9)、0.153 μg～0.765 μg(r=0.9999)范围内呈良好线性关系;二者的平均加样回收率分别为99.20%、99.26%.结论 本方法简便可行,结果可靠,可用于稀莶风湿片的质量控制.
  • 摘要：@@ 紫草Armbiae Radix,Lithosperrni Radix为紫草科多年生草本植物,始载于<神农本草经>,列为中品,以后历代本草中也都有详细记载.紫草药用其根部,性味甘、咸、寒,归心、肝经,具有凉血、活血、解毒透疹功能,主治血热毒盛、瘫疹紫黑、麻疹不透、疮疡、水火烫伤等[1].投稿日期:2010-01-06
  • 摘要：目的 通过连续3年的半夏规范化种植实验研究,制定出赫章半夏GAP规范化种植生产标准操作规程(SOP).方法 以<中药材生产质量管理规范>(GAP)为指导原则,对半夏栽培生产的区划、生态环境、栽培技术、留种技术、采收加工及包装、储运等一系列过程开展研究.结果 确定各个生产环节的最佳指标和方法,制定了半夏的规范化生产标准操作规程,提高半夏的产量和质量.结论 赫章适合半夏的规范化生产,其规范化种植标准技术成熟可行.
  • 摘要：Objective To establish a method for the content determination of honokiol in QianBeis Magnolia officinalis by HPLC. Methods A Diamonsil C18 column was used with mobile phase of methanolwater(78: 22), the detection wavelength was 294nm, the flow rate was 1.0 mL/min. Results The calibration curve showed a good linearity with the range of 0.627 2～1.164 8 μg, r=0.999 4. The average recovery was 102.12％ and RSD was 1.3％. Conclusion The method is simple, sensitive and accurate.%目的 建立高效液相色谱法测定贵州黔北凹叶厚朴中和厚朴酚的含量.方法 色谱条件:色谱柱为Diamonsil C<,18>以甲醇-水(78:22)为流动相;流速为1.0mL/min;柱温25℃;检测波长为294nm.结果 和厚朴酚线性范围为0.278 4 μg～0.649 6 μg,r=0.999 5;本品的平均回收率为102.12%,RSD为1.3%.结论 本法简便、灵敏、准确.
  • 摘要：目的 建立湿毒清胶囊中苦参碱含量的HPLC测定法.方法 C<,18>柱(4.6 mm×250 mm,5μm);乙腈-0.02%三乙胺溶液(25:75),流速为1 mL·min<'-1>,检测波长为220nm.结果 测得线性范围0.3002 μg～2.401 μg(r=0.999 9),平均回收率为97.79%,RSD为1.69%(n=5).结论 本方法简便可行,重现性好,结果可靠,可用于湿毒清胶囊的质量控制.%Objective To establish an HPLC method for the determination of matrine in Shiduqing capsule. Methods The separation was performed on a C18 analysis column (4.6 mm×250 mm, 5 tm) with acetonitrile-triethylamine ( 25: 75 ) as the mobile phase. Flow rate was 1 mL·min-1. The wavelength was 220 nm.Results There was a good linearity within the range of 0.300 2tg～2.401 6 μg(r=0.999 9). The average recovery was 97.79％, RSD=l.69％( n = 5). Conclusion This method was proved to be simple, accurate and can be used for quality control of Shiduqing Capsule.
  • 摘要：@@ 中药的替代品,或称代用品,是指与被代用药具有相同性味、归经、功能主治的药物,即不论在单方或复方中,代用品都应该与被代用药具有相同的药理作用性质和相近的作用强度,能够反映该药传统用药经验及具有临床作用的药物[1].
  • 摘要：为促进金银花种植技术在广大喀斯特地区顺利推广,促进中药材产业的顺利发展,并促进喀斯特地区生态顺利恢复,结合喀斯特地区生态恢复技术研究项目的 开展,开展了金银花主要品种忍冬在喀斯特地区的生物学特性研究、生态作用分析和规范化种植技术研究,形成了较为系统金银花规范化种植技术体系,主要包括育苗、移栽、田间管理、病虫害防治及加工等方面的内容.%In order to advance the planting techniques of Lonicerajaponica to be promoted, advance the medicine industry to be developed and advance ecological restoration in karst regions, we studied the biological characteristics and the role of ecological and standardized planting technique of Lonicera japonica in karst regions, and instituted a standardized technology system.
  • 摘要：目的 对来自10个省市的龙葵药材及其生长土壤中的16种无机元素(B、Si、V、Mn、Fe、Co、Ni、Cu、Zn、As、Se、Rb、Sr、Mo、Sn、I)进行测定和分析.方法 采用ICP-MS电感耦合等离子体质谱仪,利用SPSS统计软件对测定结果进行相关分析.结果 龙葵药材中的无机元素受土壤中的无机元素影响,8种元素(B、Mn、Fe、Co、Ni、Se、Sr、Sn)与土壤中的元素成正相关,其中Fe和sr呈显著正相关.8种元素(Si、V、Cu、Zn、As、Rb、Mo、I)呈负相关,Si呈显著负相关.自身所含的元素间也存在着一定的相关性,V、Fe、As、Se、Sr显著正相关,Co与Fe、As、Se、Sr显著正相关,与V显著负相关.结论 为科学地开发利用野生植物龙葵资源提供理论依据,并可为龙葵药材的栽培提供依据.%Objective To measure and analyze 16 kinds of inorganic elements (B, Si, V, Mn, Fe, Co,Ni, Cu, Zn, As, Se, Rb, Sr, Mo, Sn, I) in Solanum nigrum and its growth soil from 10 provinces and cities.Methods ICP-MS, SPSS statistical software were used to analyze the determination results. Results Inorganic elements in Solanum nigrum were effected by that in the soil. 8 kinds of elements (B, Mn, Fe, Co, Ni, Se, Sr,Sn) in the soil were positively correlated, in which Fe and Sr were significantly correlated. 8 kinds of elements (Si, V, Cu, Zn, As, Rb, Mo, I) were negatively correlated, Si was significantly negatively correlated. Among the elements of self-contained there is some correlation, V, Fe, As, Se, Sr was a significant positive correlation, Co was significant positively correlated with Fe, As, Se, Sr, and negatively correlated with V. Conclusion Provide the basis for scientifically developing and utilizing wildlife resources of Solanum nigrum and its cultivation.
  • 摘要：UV spectrum, HPLC and other methods were used to study the differences of white edible bird's nest and red one, The test results showed that there were some differences between the two edible bird's nest on UV patterns,HPLC patterns, obviously on character and the iron content. It can be easily identified the red bird's nest which was dyeing or not by using methods of this paper%分别利用紫外光谱、铁含量及唾液酸含量HPLC分析等方法,研究燕窝中白燕及血燕的差别.结果 表明白燕和血燕在紫外谱图和液相谱图上有一定差别,在性状和铁含量上有较大差别.通过这些指标成分分析,可以鉴别白燕通过染色冒充血燕的产品.
  • 摘要：Objective To research the effective parts of anti-rheumatoid arthritis of Daphne giraldii Nitsche. Methods The different parts of the Daphne giraldii Nitsche. were extracted and separated by the methods of system solvent extraction. Setting anti-inflammatory analgesic and irritating as indicators, the effective part of rheumatoid arthritis and site of adverse reactions were determined by the animal experiment.Results Ethyl acetate extract and n-butanol extract were effective pans of anti-rheumatoid arthritis, and irritating parts of Daphne giraldii Nitsche. was mainly from petroleum ether extract. Conclusion The effective parts of anti-rheumatoid arthritis of Daphne giraldii Nitsche. was identified initially, which provided the foundation of new dosage.%目的 研究祖师麻抗类风湿性关节炎的有效部位.方法 采用系统溶剂提取法对祖师麻进行提取、分离得到不同部位的提取物.以抗炎镇痛、刺激性等为指标,通过动物实验确定了祖师麻治疗类风湿性关节炎的有效部位及不良反应的部位.结果 乙酸乙酯提取物和正丁醇提取物为祖师麻抗类风湿关节炎的有效部位,刺激性主要来源于石油醚部位.结论 初步确定了祖师麻抗类风湿关节炎的有效部位,为祖师麻开发成新剂型奠定了基础.
  • 摘要：Objective To study the anti-inflammatory and skin irritation of Meile plaster. Methods Using the ironware to make deep second degree bum model and determine the content of serum IL- 1, IL-6, TNF alpha;observing the irritation after single-dose administration. Results High, medium dose group can decrease the content of serum IL-l, IL-6, TNF alpha after scalding 6 h, 24 h; compared with the blank control group there was significant difference ( P＜0.05 ) ; there was no irritation after single-dose administration to intact skin and damaged skin. Conclusion Meile plaster was anti-inflammatory and safe for single-dose administration.%目的 研究美乐涂膜剂的抗炎作用及单次给药对皮肤的刺激性.方法 采用铁器烫伤制作深Ⅱ度皮肤烫伤模型,测定血清IL-1,IL-6,TNF-α含量;采用完整皮肤、破损皮肤单次给药,观察其刺激性.结果 高、中剂量组能够明显降低大鼠烫伤6 h、24 h后血清中IL-1,IL-6,TNT-α含量,与赋形剂组比较有显著性差异(P<0.05);单次背部给药,对动物完整皮肤、破损皮肤未产生任何刺激反应.结论 美乐涂膜剂具有抗炎作用及单次用药的安全性.
  • 摘要：目的 优选出化风丹中有效成分天麻素的最佳提取方法.方法 本文采用回流法和超声法提取,以HPLC测定天麻素含量为指标,对提取条件进行优选.结果 最佳提取条件为:氯仿40 mL,超声提取40 min,弃去氯仿液,药渣挥尽氟仿,加甲醇40 mL,超声40 rain.结论 优选的提取方法可行,省时,具有可操作性.%Objective To study the extraction of Gastrodin.Methods Using reflux and ultrasonic to extract and the content of Gastrodin was determined by HPLC to optimize the extracting condition.Results The optimal condition was as follows: adding chloroform 40mL to extract by ultrasonic for 40min, then filter the chloroform,adding methanol 40mL extracted for 40min again.Conclusion The optimal method was feasible, effective and had the operability.
  • 摘要：As a traditional medicine,Gymnema sylvestre has wide distribution and large quantity in China.Recently, There have been many researches on the effect of GS,including antidiabetic properties,controlling obesity, etc.The main active compound of the plant is a group of triterpene saponins termed as gymnemic acids,the hypoglycemic mechanism is that can stimulate insulin secretion, reduce blood lipid and inhibite intestinal glucose absorption. This review will try to put forth an overall idea about the effect of GS, the active components and the compouned recipe on diabetes mellieus.%匙羹藤作为一种传统民间药物,在我国分布广.近年来国外学者己对其作了多方面的研究,发现它具有防治糖尿病的作用,其降糖的有效成分主要是命名为匙羹藤酸的一系列三萜皂苷类化合物和牛弥菜醇A,降糖机制主要有促胰岛素分泌、保护胰岛B细胞、增加外周葡萄糖的利用和抑制肠道葡萄糖的吸收.本文从匙羹藤单方、活性成分及其复方在防治糖尿病方面的作用及其机制研究做一概况.
  • 摘要：目的 研究三叶青扦插育苗技术.方法 研究了生根剂、插穗年龄和基质对插穗生根的影响.结果 生根剂、插穗年龄对插穗生根的影响明显,基质对插穗生根的影响不明显.结论 2～3年生老枝1 000mg/LIBA浸溃10s后扦插于黄土中生根效果最佳.%Objective To study the cutting technique of Tetrastigmatis hemsleyani. Methods The effects of concentrations of rooting reagents, cutting age and cutting medium were investigated. Results The rooting reagents and cutting age effected rooting rate obviously, but cutting medium had no obvious effect on the rooting rate. Conclusion The best combination of factors was as follows: 2-3 age cutting treated by IBA (1 000 mg/L)for 10s and planted in the cutting medium of Loess.
  • 摘要：@@ 毛黄堇Corydalis tomentella Franch.系罂粟科紫堇属植物,又名干岩矸(重庆南川)、岩黄连(湖北巴东、陕西镇坪)[1],为民间常用中草药.具有抗菌、消炎、镇痛和强安定作用,此外还有抑制肿瘤细胞作用.
  • 摘要：目的 建立燕窝及冰糖燕窝的鉴别方法,为制定其质量标准提供依据.方法 采用现代显微技术,时目前市场流通领域的燕窝及冰糖燕窝进行显微等鉴别;薄层色谱以五种氨基酸为对照品,以正丁醇-冰醋酸-水(4:1:1)为展开剂,喷以茚三酮试液,在105℃加热检视.结果 燕窝及冰糖燕窝在显微特征上基本相同;燕窝及冰糖燕窝在对照品相同位置上有相同颜色的斑点.结论 本研究结果可以作为燕窝及冰糖燕窝真伪鉴别的依据.
  • 摘要：@@ 我国已报道的柴胡有42种、17变种及7个变型,其中作为药用的有25种、8变种、3变型[1].柴胡为清虚热中药,用于感冒发热、肝郁气滞、月经不调、胸胁疼痛、气虚下陷、久泻脱肛等症,为我国常用大宗中药材品种之一,不仅国内需要量大,而且大量出口,年需要量4 000吨以上.
  • 摘要：Objective To discuss the influence ot drug compatibility on the components ot the volatile oil in Frankincense and Myrrh. Methods GC-MS system was used to analyze the volatile oil in Frankincense,Myrrh and their combination. The effect of compatibility on the components and content of volatile oil was evaluated. Results The results of GC-MS showed that cadina-1 ( 10 ) ,4-diene, yl-2H-1-Benzopyran, 3,5,6,8 a-tetrahydro-2,5,5,8 a-tetramethyl-, trans- was detectable in Frankincense but was undetectable in the drag pair; Phenol,2-(1,2-dimethyl-2- cyclopenten-l-yl)-, acetate, Naphthalene, 1,2,3,4-tetrahydro-l,6-dimethyl-4 -(1-methylethyl)-,(1 S-cis)-, Azulen-2-ol, 1,4-dimethyl-7-(l-methylethyl)- was detactable in Myrrh but was undectable in the drug pair;1-ethenyl -1-methyl - 2,4-diisopropyl -cyclohexane, 1,2,3,4- tetrahydro-1-methyl -8-Isopropylnaphthalene was dectable in the drug pair but was undetectable in the two single drugs. Conclusion The compatibility of the drug pair has an effect on components and contents in the volatile oil of Frankincense and Myrrh.%目的 考察乳香、没药药对配伍对挥发油化学成分的影响.方法 采用气-质联用系统对乳香、没药药材以及药对配伍中的挥发油进行分析,评价配伍对挥发油成分及含量的影响.结果 采用GC-MS对乳香、没药药材和药对挥发油的成分进行分析比较,发现乳香挥发油中检出的trans-3,5,6,8 a-四氢-2,5,5,8a-四甲基-y1-2H-1-苯并吡喃、荜澄茄-1(10),4-二烯等成分和没药挥发油中检出的2-(1,2-二甲基-2-环戊烯-1-y1)乙酸苯酯、(1S-cis)-1,2,3,4-四氢-1,6-二甲基-4-(1-甲基乙基)-萘、1,4-二甲基-7-(1-甲基乙基)-Azulen-2-醇、(-)-匙叶桉油烯醇等成分在药对挥发油中未检出,而相对于单煎所得的挥发油,药对挥发油中新增成分有1-乙烯基-1-甲基-2,4-二异丙基-环己烷、1,2,3,4-四氢-1-甲基-8-异丙基萘等.结论 乳香、没药药对配伍对所提挥发油的成分和含量都有较大的影响.
  • 摘要：Objective To study the molding process of night-ning capsule. Methods Survey physical characteristics of the dry powder and the particle including hygroscopicity, angle of repose and bulk density,respectively. Results The dry powder has major hygroscopicity and minor angle of repose, and particle has minor fluidity. The hydroscopicity and angle of repose can be improved by granulation which can make the capsule loading easier. Conclusion The extracts of night-ning capsule is suitable to make the particle, and the yield of capsule is increased after improving the particle physical characteristics.%目的 研究夜宁胶囊的成型工艺.方法 分别考察干粉的粉体及颗粒的物理特性(吸湿率、休止角及堆密度等).结果 干粉的吸湿性较大、休止角较小;颗粒的流动性较差;通过制粒可以改善粉体的引湿性及休止角,更有利于装胶囊.结论 夜宁胶囊提取物宜制成颗粒,改善粉体物理特性后进行灌装胶囊,提高成品率.
  • 摘要：目的 建立同时测定木香槟榔丸中大黄素和大黄酚含量的方法.方法 采用HPLC法,色谱条件为LunaC<,18>色谱柱(250mm×4.6 MM,5 μm),流动相为乙腈-0.1%磷酸溶液(81:19),流速为1.0mL·min<'-1>,柱温:30℃,检测波长为254 nm.结果 大黄素和大黄酚分别在2.52～50.40 μg·mL<'-1>(r=0.999 7)和0.019～0.380 mg·mL<'-1>(r=0.999 8)浓度范围内均有良好的线性关系,平均加样回收率分别为98.2%,97.2%,RSD均小于2.0%.结论 本方法简便,快速,准确,重复性好,可用于该制剂的质量控制.%Objective To establish a method for the determination of emodin and chrysophanol in Muxiang Binglang Wan. Methods HPLC system consisting the Luna C18 ( 250 mm×4.6 mm, 5 μm ) column, the mobile phase consisted of Acetonitrile -0.2％H3PO4 (81: 19), the flow rate was 1.0 mL·min-1, the column temperature was 30℃, UV detector was set at 254 nm. Results The linear response range was 2.52-50.40 μg·mL-1 ( r=0.9997 ) and 0.019-0.380 mg·mL-1 ( r=0.999 8 ) , respectively. The average recovery of emodin and chrysophanol were 98.2％ and 97.2％, respectively, and RSD ＜ 2.0％. Conclusion The method was proved to be simple and accurate, repeatable, it can be used for the quality control of Muxiang Binglang Wan.
  • 摘要：目的 研究制定痛经灵颗粒的质量标准.方法 用薄层色谱法定性鉴别赤芍、香附、元胡、桂枝和红花;用高效液相色谱法测定原儿茶醛的含量.结果 原儿茶醛在0.169～1.123 μg范围内呈良好的线性关系,r=0.9997,平均回收率为99.5%,RSD=0.83%.结论 鉴别方法专属性强,定量方法简便、准确、可靠,可用于该制剂的质量控制.%Objective To research the quality control standard of Tongjingling Granule. Methods Red Peony Root, Cyperi Rhizoma, Corydalis Rhizoma, Cinnamom Ramulusi and Carthami Flos were identified in Tongjingling granules by TLC. The protocatechuicaldehyde content in Tongjingling granules was determined by RP-HPLC, with methylalcohol-0.5％acetic acid ( 20:80 ) as molile phase, the detection wavelength was set at 280 nm and flow rate was 1.0 mL-min-1. Results Protocatechuicaldehyde showed a good lineafity in a range of 0.169～1.123 μg(r=0.999 7).The average recovery of protocatechuicaldehyde was 99.5％ and RSD was 0.83％(n=5).Conclusion The method is simple, specific and accurate. This study provide a method for the quality control of Tongjingling granules.
  • 摘要：目的 对贵州省野生和栽培共24个天麻基因组DNA的遗传多态性进行分析,构建其SSR指纹图谱.方法 采用改良的CTAB法提取天麻基因组DNA,采用NTSYSpc软件进行UPGMA聚类分析和遗传相似度分析.结果 改良的CTAB法能够获得纯度和浓度较高的天麻基因组DNA,PCR扩增获得较清晰的SSR指纹图谱,UPGMA聚类可明显区分野生与栽培天麻.结论 SSR指纹图谱的UPGMA聚类分析表明野生天麻和栽培天麻遗传相似度较接近,栽培天麻产生较大变异.%Objective Get genomic DNA from Genetic Gastrodia elata Blume. for PCR, analyzed genetic polymorphisms of genomic DNA from 24 samples of wild and cultivated Gastrodia elata Blume. in Guizhou, set up the SSR fingerprintings. Methods DNA was obtained by CTAB methods, and 10 primers from Genebank were selected for SSR-PCR, UPGMA dendrogram was analyzed by NTSYSpc with SSR fingerprintings.Results Genetic DNA obtained by CTAB mehods with high purity and cencetration was fit for PCR, got 3 primers from 10 and clear fingerprintings, the similarity coefficient of the fingerprintings can obviously distinguish the wild and cultivated Gastrodia elata Blume.. Conclusion UPGMA dendrogram of SSR showed that wild and cultivated Gastrodia elata Blume. could be distinguished from each other, but cultivated one showed the great genetic viaration.
  • 摘要：Objective To investigate resources of Atracty lodesmacrocephala Koidz. and processing in producting area from Bozhou district in Anhui province. Methods By means of the field survey. Results Planting of A.lode macrocephala Koidz. get develops rapidly, total planting area reached to 3 300 hm in Bozhou district of Anhui province and it occupied the main market share currently in our country. Conclusion To ensure the quality of A.lode macrocephala which should be paid great attention to study of artificial cultivation of A. lode macrocephala.%目的 调查安徽省亳州地区白术资源和产地加工情况.方法 实地调查.结果 安徽省亳州地区白术生产发展迅猛,总种植面积达5万亩(3300 hm),已占白术市场的主要份额.结论 重视人工栽培白术的研究,保证白术品质,对于促进安徽经济发展和开发安徽道地药材,具有重要意义.
  • 摘要：目的 筛选唇香草抑菌活性成分及分析其挥发油类化学成分.方法 采用纸片琼脂扩散法和对倍稀释法对唇香草水提取物与挥发油进行抑菌实验研究,经GC-MS联用技术,结合Kovats指数技术对唇香草挥发油抑菌的活性成分进行了成分分析.结果 唇香草挥发油对白假丝酵母菌有较强的抑制作用,水提取物对金黄色葡萄球菌有较强的抑制作用,而对大肠杆菌、铜绿假单胞菌的抑制作用较弱.结论 唇香草挥发油和水提取物对白假丝酵母菌、金黄色葡萄球菌有较好抑制作用,本研究为唇香草的临床应用提供了理论根据.%Objective To study inhibiting bacterial activity and volatile oil chemical composition analysis of Ziziphora clinopodioides Lam. Methods Different extraction of the Ziziphora clinopodioides Lam inhibited bacterial activity screening by the filter paper method and standard plate dilution method. In order to find antibacterial components, the constituents of volatile oil from Ziziphora clinopodioides Lam. were analysed by techniques of GC-MS and Kovats index. Results Volatile oil of Ziziphora clinopodioides Lam. has a stronger effect on Candida albicans, aqueous extract has a good effect on Staphylococcus aureus and a weak effect on Escherichia coli, Pseudomonas aeruginosa. Conclusion The volatile oil and extract of water have a better effect for inhibting Candida albicans, Staphylococcus aureus. This study provide theory basis for clinical treatment of Ziziphora clinopodioides Lam.
  • 摘要：Objective To study the optimum extraction process of the total flavonoids and phenolic acid and caffeic acid from the Lycopi Herba.Methods The effect of the concentration of ethanol, extraction time duration, extraction time and alcohol amount were investigated by the orthonogal design with the content of tiliroside as the chemical reference.Results The optimum extraction condition was established as follows: with 15 times ethanol concentration 50％, extracting 2 hours for three times.Conclusion The method is simple and feasible, and is the optimum extraction process of the total flavohoids, phenolic acid and caffeic acid from Lycopus Lucidus.%目的 研究泽兰总黄酮及酚酸类成分的最佳提取工艺.方法 以总黄酮及酚酸、咖啡酸舍量为指标,采用L<,9>(3<'4>)正交试验,考察提取溶媒、提取次数、提取时间、溶媒用量因素时提取的影响,确定泽兰最佳提取工艺.结果 以15倍量的50%乙醇为溶媒,回流提取3次,每次2 h为最佳提取条件.结论 本提取工艺方法简单、合理,是泽兰黄酮及酚酸类成分的最佳提取工艺.
  • 摘要：目的 优选金荞麦抗癌有效成分最佳提取工艺.方法 采用正交实验,考察了溶剂种类、溶液pH值、提取温度、提取时间对金荞麦中抗癌成分提取量的影响.采用紫外分光光度法测定提取物中总原花青素含量,HPLC法测定提取物中原花青素B2的含量.结果 对总原花青素及原花青素B2提取溶剂种类、溶液pH值、提取温度是主要影响因素,均具有统计学意义.结论 确定金荞麦抗癌成分提取最佳工艺为:溶剂为50%乙醇,pH为7(原溶液pH值),温度65℃,提取3次,每次1 h.%Objective To study the optimum extraction technology of anti-cancer ingredients of Fagopyri Dibotoryis Rhizoma. Methods The concentrations of anti-cancer ingredients of Rhizoma Fagopyri Dibotoryis were used as estimated indexes. Solvent type and pH, extraction temperature, time were investigated by orthogonal experiment. The total procyanidins was analysised by UV. Procyanidin dimer B2 was analysised by HPLC.Results Solvent type and pH, extraction temperature are the leading influencing factors, as there are remarkable difference between each level. Conclusion The best extraction technology is as follows: 50％ alcohol, pH=7 (the original solution pH),at 65 ℃, extracting for 3 times, 1 h each time.

    现代中药研究与实践的期刊信息

    • 曾用名：基层中药杂志
    • 创刊时间：1987
    • 地区：CN
    • 语言：中文
    • 热门主题：安徽中医药高等专科学校
    • 学科分类：

    现代中药研究与实践的获奖情况

    • • 获1998年安徽省优秀期刊

    现代中药研究与实践的收录情况

    • 中国·中国科技核心期刊
    • 中国·中国人文社科核心期刊
    • 中国·武大RCCSE核心期刊（2020）
    • 美国·化学文摘（网络版）

    现代中药研究与实践的投稿信息

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    • 期刊栏目：研究论文；药品质量；中药鉴定；资源利用；药材栽培；中药制剂；中药炮制；综述杂志
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