八角茴香提取物抗氧化活性分析

摘要

Star anise ( lllicium verum Hook. f. ), an important source of natural anti-oxidants, was extracted with solvents of water, ethanol, actone and n- butanol, respectively. The extracts were determined in free radical scavenging activity by using 2,2-diphenyl-l-picrylhydrazyl (DPPH) assay. The volatile oil and the ethyl acetate extract from these extracts were used as antioxidants and added into the lard quantitatively, and the peroxide val- ue (POV) of the lard stored for a given time was measured to compare the influence of different rates of the vola- tile oil and the ethyl acetate extract on oxidation resistance of the lard. The results showed that the extracts varied in free radical scavenging activity which was the highest in the ethanol extract with the order of ethanol 〉 n- buta- nol 〉 actone 〉 water under the same concentration. The volatile oil was low in anti-oxidative activity while the ethyl acetate extract had very high anti-oxidative activity. The ethyl acetate extract was higher in oxidation resist- ance when added into the lard at a rate of 0.10% (w) than the butylated hydroxytoluene (BHT) at a rate of 0. 02% (w), and had higher anti-oxidative activity when added at a higher rate.%摘要：以蒸馏水、乙醇、丙酮、正丁醇为溶剂对八角茴香进行提取，采用DPPH法对各提取物进行自由基清除实验，同时，以挥发性油和乙酸乙酯提取物作为抗氧化剂，定量加入油脂中，通过对强化保存期间过氧化值（POV）的测定，比较它们不同的添加量对猪油抗氧化性能的影响。结果表明，同一质量浓度不同溶剂的提取物对DPPH自由基的清除能力作用强弱是：乙醇提取物〉正丁醇提取物〉丙酮提取物〉蒸馏水提取物；挥发性油抗氧化效果不明显，乙酸乙酯提取物具有很强的抗氧化活性，当乙酸乙酯提取物添加量达w=0．10％时，抗氧化效果优于w=0．02％的BHT，且添加量越高抗氧化效果越好。