高效液相色谱-串联质谱法测定六堡茶中黄曲霉毒素B1的研究

摘要

Objective Applied in modified QuEChERS method with nano Bamboo Charcoal(NBC) as sorbents ,to establish a determination method for the Aflatoxin B1 components in Liubao tea by HPLC-Tandem Mass Spectrom-etry . Methods AFB1 in Liubao tea were extracted with acetonitrile ,The extrat was cleaned up using the mixture of NBC-PSA(primary secondary amine)-MgSO4 in dispersive solid-phase step . Results The calibration curve showed a good linearity in the range of 2.6~41.6μg/kg .The recoveries of AFB1 in Liubao tea were 83.8% ,and the relative standard deviations (RSD ,n=10) were 4.7% .The quantification (LOQ) limits were 2.6 μg/kg . Conclusion The method could meet the requirements for Aflatoxin B1 analysis in Liubao tea .%目的 建立以多壁碳纳米管作为 QuEChERS 吸附剂 ,高效液相色谱-串联质谱(UPLC-MS/MS)法测定六堡茶中黄曲霉毒素B1 的分析方法.方法 采用乙腈提取 ,并在样品提取液中加入分散固相基质净化后进行测定.结果 在 2.6 ~41.6 μg/kg浓度范围内具有良好线性关系 ,黄曲霉毒素B1 的回收率为83.8% ,相对标准偏差(RSD )4.7% ,n= 10 ,方法检测限为2.6 μg/kg.结论 该法有较好的灵敏度 ,能够满足六堡茶中黄曲霉毒素B1 测定的要求.