An Arabidopsis male sterile mutantshrunken pollen grain1,spg1 was obtained through enthyl methane sulfona (EMS) mutagenesis strategy.By the observation of the transverse section,the mutant showed the aberrant microspores and pollen grains compared with wild type.Genetic assay indicated that spg1 phenotype was controlled by a single recessive mutant locus,and this locus was mapped to the region about 427kb flanked with genetic marker 3 - AL138638 - 6632 and 3 -AL353865 -6814 on chromosome 3 by map-based cloning approach.Bioinformatics analysis showed there were no known male sterile genes in this region.It indicated SPG1 was a new male sterile gene in Arabidopsis.%通过EMS诱变获得一个拟南芥突变体shrunken pollen grain1,spg1.根据横切片的观察结果,与野生型相比较,突变体观察到异常的花粉粒.遗传学的实验表明:spg1突变现象由一个隐性的单基因控制.通过图位克隆的方法,spg1突变位点定位在3号染色体的分子标记3 - AL138638 -6632和3- AL353865 -6814之间大约427kb的区间内.生物信息学的分析表明,在这个区间内没有任何已知的与育性相关的基因.以上结果说明SPG1是一个控制拟南芥雄性不育的新基因.
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