miR-96-5p在肝细胞癌根治术后早期复发患者中的表达及其临床意义

摘要

Objective To investigate the expression and clinical significance of miR-96-5p in primary hepatocellular carcinoma (HCC) at early recurrence after radical surgery. Methods 61 HCC eryopreservation tissue samples from the liver carcinoma specimens data obtained after radical surgery and banked in our hospital were divided into 2 groups: early recurrence group (33 cases) and non-early recurrence group (28 cases). Aquantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression of miR-96-5p. Results Compared with the non-early recurrence group , the expression of miR-96-5p was observably down-regulated [(0.634 ± 0.783) vs (5.182 ± 11.321), P = 0.043]. The expression of miR-96-5p was correlated to tumor diameter, early recurrence and vascular invasion (P<0.05). Conclusions miR-96-5p are significantly related to early liver cancer recurrence and metastasis. miR-96-5p may be a molecular marker of HCC at early recurrence as well as a target for targeted therapy of liver cancer in future.%目的：探讨miR-96-5p 在肝细胞癌根治术后复发患者中的表达及其临床意义。方法：从我院肝癌标本库中选取61例冻存的原发性肝细胞癌组织标本，根据筛选标准将其中33例归为早期复发组（根治术后1年内出现肝内复发病灶），28例为非早期复发组（术后超过2年未出现肝内复发病灶）；运用实时荧光定量PCR 验证miR-96-5p 在两组肝细胞癌组织的表达水平。结果：miR-96-5p 在早期复发组肝癌组织中表达明显下调[（0．634±0．783） vs．（5．182±11．321），P＝0．043]，其在肝癌组织中的表达与肝癌术后早期复发及肿瘤直径、脉管癌栓有关（P＜0．05）。结论：miR-96-5p 与肝癌根治术后早期复发密切相关，并极有可能成为肝癌早期复发的分子标记物，以及为未来肝癌靶向治疗提供有效的靶点。