大豆乳清蛋白发泡特性及辅助抑制肿瘤作用研究

摘要

为考察大豆乳清蛋白发泡特性及辅助抑制肿瘤作用,采用截留分子质量(MWCO)为10 000 u再生纤维膜(RXC)进行试验,考察大豆乳清蛋白发泡特性及其最佳控制条件,同时进行辅助抑制肿瘤作用试验,考察经口给予小鼠不同剂量的大豆乳清蛋白30 d(接种S-180腹水型肿瘤细胞),对小鼠体重、脏器/体重和移植S-180瘤株肿瘤生长的影响.结果表明,浓度、温度、pH、时间和阳离子强度均对大豆乳清蛋白起泡性有显著影响.大豆乳清蛋白起泡性效果最佳条件是:浓度1.O%,温度60℃,pH 5,钙离子强度0.1 M.经口给予小鼠不同剂量的大豆乳清蛋白30 d,对小鼠体重增长和脏器体重比值无影响,对小鼠S180肿瘤的生长有抑制作用,说明大豆乳清蛋白具有辅助抑制肿瘤的作用.显微镜观察结果表明1.6 g·L-1和2.0 g·L-1浓度的大豆乳清蛋白处理人肝癌细胞HepG2 48 h后,细胞核或细胞质呈浓染的块状或颗粒状荧光,聚集于核周边或裂解成碎片,表明大豆乳清蛋白能诱发肿瘤细胞发生细胞凋亡.%In order to inspect whey soy protein foaming characteristics and its adjuvant action on inhibiting and preventing the growth of tumor, the molecular weight cut-off (MWCO) for 10 000 u of regenerated fiber membrane (RXC) was used to inspect whey soy protein foaming characteristics and its best foam control conditions, at the same time, the experiment of adjuvant inhibiting and preventing the growth of tumor was carried out. The experiment was carried out by giving different doses of whey soy proteins to mice through their mouths for 30 d, (inoculated S-180 ascites tumor cells), in order to inspect the effects of weight, the ratio of organ and weight, and the growth of transplant S-180 ascites type tumor cell on mice. The results showed that: the concentration, temperature, pH, time and cationic intensity had a significant influence on soybean whey proteins foaming. The optimum condition of whey soy proteins foaming condition should be 1.0% concentration, 60℃ temperature, pH 5 and 0.1 mol·L-1. Calcium ionic strength. Feeding mice through their mouths in different doses for 30 d, which showed that there was no obvious influence on the growth of weight and the ratio of organ and weight of giving whey soy proteins of mice, but there was the inhibition role of soy whey proteins on the S-180 ascites type tumor cell, which indicated that whey soy proteins could inhibit the growth of tumor. Microscopy results showed that using the concentration of 1.6 g·L-1 and 2.0 g·L-1 whey soy proteins to deal with human hepatocellular carcinoma cell HepG2 48 h later, the nucleus or cytoplasm presented chromatoid massive or granular fluorescence that aggregated around call nuclear or became pieces. It indicated that whey soy proteins could induce the apeptosis of tumor cell.