Objective To investigate the free radical mechanism in brain of rats involved in the injury induced by cerebral ischemia - reperfusion. Methods The middle cerebral artery occlusion (MCAO) was occluded with thread according to modified Zea longa's method. Wistar rats were randomly divided into; sham - operation group ( sham ), ischemia - reperfusion group (IR) 6h, 1day, 2days, 3days,7days. The content of superoxide dismutase( SOD) and malondialdehyde ( MDA) in brain tissues were measured by comparative methods. Results The activity of SOD and the content of MDA in brain were determined respectively. The activity of SOD decreased immediately after operation,and came to the lowest point after 24h,then increased in the following time,whereas the leaves of MDA came to the highest point after 2 days,then decreased. There was significance difference between sham group and I/R group. Conclusion The activity of SOD decreased after operation, then increased in the following time, whereas the levels of MDA came to the highest, then decreased. They have negative correlation. The lycopene can reduce the content of MDA after focal cerebral ischemia - reperfusion injury,can lighten the level of vasogenic brain edema, and improve the neurological defieit scores, so it has a protective effect on impaired neuron in cerebral ischemia - reperfusion injury.%目的 探讨大鼠血清中SOD活力和MDA含量在脑缺血再灌注(CIRP)损伤过程中的变化以及番茄红素干预后的影响.方法 采用线栓法制作实验大鼠大脑中动脉缺血再灌注模型( MCAO).将Wistar大鼠随机分为:假手术组、缺血再灌注组(I/R组)(6h、1天、2天、3天、7天组)和番茄红素(LP组).运用比色法检测大鼠血清中SOD和MDA.结果 假手术组中SOD活力高表达、MDA低表达；I/R组的血清中SOD活力在各时间点均低于假手术组,并且在1天显著降低,2天SOD活力降到最低；MDA含量在6h开始升高,1天明显升高,2天达高峰；LP组从CIRP后血清SOD活力明显高于I/R组,MDA含量明显低于L/R组.结论 大鼠血清中的SOD、MDA参与了CIRP损伤,番茄红素对大鼠CIRP后发生的氧化损伤有干预作用.
展开▼
