鹿茸提取物对顺铂诱导人胚肾细胞损伤的影响∗

摘要

This experiment was designed to study the effects of pilose antler extractions ( water ex⁃tract, ethanol extract, ether extract, chloroform extract and ethyl acetate extract) to protect HEK293 injury induced by cisplatin( CDDP ) . HEK293 was cultured in vitro and the results were determined by MTT and LDH. Pilose antler extractions can protect CDDP⁃induced injury of HEK293, and the death rate was more than half at the level of (0�083±0�030) mmol/L. After HEK293 cells were in⁃cubated for 24 h with different concentrations of extract of pilose antler, CDDP was joined respec⁃tively. It was found that water extraction has better protection on CDDP⁃induced HEK293, and at the level of 1 mg/mL, the survival rate of cells was 62%( P<0�01) . Water extraction can effectively protect CDDP⁃induced HEK293 and maintain cell activity.%建立顺铂（CDDP）诱导人胚肾成纤维细胞（HEK293）细胞损伤模型，通过MTT和LDH测定，比较不同浓度的鹿茸提取物（水提物，醇提物，乙醚提取物，氯仿提取物，乙酸乙酯提取物）诱导HEK293细胞损伤的保护作用。体外培养HEK293细胞，观察鹿茸提取物对CDDP诱导HEK293细胞生长的影响，并用MTT法和LDH法检测鹿茸对CDDP诱导HEK293细胞死亡率的变化。结果表明：鹿茸提取物能够拮抗CDDP诱发的细胞损伤，具有浓度依赖性，其半数致死浓度为（0�083±0�030） mmol／L。当HEK293细胞与不同浓度鹿茸提取物共同孵育24 h，分别加入CDDP后，检测表明鹿茸水提物较其他溶剂提取物对顺铂诱导损伤的HEK293有较好的保护作用，其质量浓度为1 mg／mL时对顺铂诱导损伤的HEK293细胞的保护作用最强，细胞存活率达到62�0％（ P＜0�01）。鹿茸水提物对CDDP诱导人胚肾细胞损伤的抑制作用最显著，可有效保护细胞活性。