7种牛奶蛋白基因在大肠杆菌中的异源表达

摘要

通过将牛奶中的αs1-酪蛋白、αs2-酪蛋白、β-酪蛋白、κ-酪蛋白、α-乳清蛋白、β-乳球蛋白及白蛋白共7种主要蛋白基因在大肠杆菌中进行了异源表达，并对其中的三种乳清蛋白进行了 Western-blot验证。研究结果显示，大肠杆菌可成功表达7种牛奶蛋白，且未被降解。这表明将大肠杆菌作为产生重组牛奶蛋白的底盘细胞具有较好的应用潜能，以上研究对后续进一步开发人造牛奶奠定了基础。%Seven main cow milk protein genes, includingαs1CN,αs2CN,βCN,κCN,αLa,βLg, and BSA (Bovine Serum Albumin), were expressed inEscherichia coli and three whey proteins were examined by western-blot. As a trial substage, these genes of seven cow milk protiens expressed successfully inE. coli wihout a phenomenon of degradation. The results indicated that it is initial feasible to gain recombinant milk proteins using recombinantE. coli, and this work lays foundations for the production of cow free milk.