VTvaf17-Act1-Cas9 GENE-THERAPEUTIC DNA VECTOR BASED ON VTvaf17 GENE-THERAPEUTIC DNA VECTOR CARRYING TARGET Cas9 GENE FOR HETEROLOGOUS EXPRESSION OF THIS TARGET GENE IN PLANT CELLS DURING GENOME EDITING OF PLANTS, METHOD OF PRODUCING AND USING GENE-THERAPEUTIC DNA VECTOR, STRAIN OF ESCHERICHIA COLI SCS110-AF/VTvaf17-Act1-Cas9 CARRYING GENE-THERAPEUTIC DNA VECTOR, METHOD FOR PREPARING THEREOF, METHOD FOR INDUSTRIAL PRODUCTION OF GENE-THERAPEUTIC DNA VECTOR

摘要

FIELD: biotechnology; medicine; agriculture.;SUBSTANCE: invention relates to genetic engineering and can be used in biotechnology, medicine and agriculture to create plant varieties, including transgenic plants. Invention is a VTvaf17-Act1-Cas9 genetherapeutic DNA vector based on the VTvaf17 gene-vector DNA vector carrying the target Cas9 gene for heterologous expression of the target gene in plant cells during genome editing of plants, wherein the VTvaf17-Act1-Cas9 gene-therapeutic DNA vector has nucleotide sequence SEQ ID No. 1.;EFFECT: invention can be used in genome editing of a gene in plant cells.;8 cl, 7 dwg, 9 ex