纤维素酶产生菌的筛选及酶学性质研究

摘要

Isolated Cellulose decomposing ability was the strongest from city life garbage, and extracellular enzyme production strains, tentatively identified as Bacillus ,and enzymatic properties of cellulase produced by the bacteria were investigated. The cellulase reaction, the optimum temperature is 50℃ ; the optimum pH7.0; enzyme at 40~50℃ has good thermal stability; the activity of CMCase in pH 6 ~ 7 can be maintained above 70%, the activity of FPA in pH 6~7 can be maintained more than 79%; Ca2+ and Mg2+ for the enzyme reaction were increased markedly, While Fe3+ and Mn2+ had inhibitory effect on the enzyme reaction, effect of Na+, Zn2+ and K+ on the enzyme is very small.%从城市生活垃圾中分离到一株分解纤维素能力最强，并产生胞外酶的菌株，初步确定为芽孢杆菌，并对该菌所产的纤维素酶的酶学性质进行了初步研究.该纤维素酶反应最佳温度为50℃；最适 pH7.0；酶在40~50℃热稳定性较好； CMCase活力在 pH 6.0~7.0处可保持70%以上， FPA酶活在pH 6.0~7.0处可保持79%以上； Ca2+和Mg2+对酶反应表现为明显的促进作用，而Fe3+和Mn2+对酶反应有抑制作用， Na+、Zn2+和K+对酶的影响很小.